| 响应面法优化猪血浆蛋白制备铁螯合肽的酶解工艺 |
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| 引用本文： | 朱峰,郭俊,范翠玲,朱梅,朱林,赵敏,朱晓梅,王瑞,罗海蓉,王建影,李玉喜,宋文峰,谢翠平,谢宁宁. 响应面法优化猪血浆蛋白制备铁螯合肽的酶解工艺[J]. 农业科学与技术, 2017, 18(7) |
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| 作者姓名： | 朱峰 郭俊 范翠玲 朱梅 朱林 赵敏 朱晓梅 王瑞 罗海蓉 王建影 李玉喜 宋文峰 谢翠平 谢宁宁 |
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| 作者单位： | 安徽珠峰生物科技有限公司,安徽太和,236600 |
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| 摘 要： | 应用响应面法对猪血浆蛋白酶解制备铁螯合多肽的工艺进行优化,在单因素试验的基础上,选择pH值、温度、时间为影响因素,水解度为指标,进行三因素三水平的Box-gehnken中心组合试验设计,采用响应面法分析3个因素对响应值的影响 结果表明,制备蛋白粉最佳工艺条件为:pH值为7.7,温度为46.3℃c.时间为7.4 h,在该条件下,蛋白水解度的为35.52％,水解物螫合率为79.41％.该]工艺可为猪血浆蛋白开发利用提供新的思路.
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| 关 键 词： | 响应面法 猪血蛋白 酶解 水解度 |
Optimization of Enzymolysis Process of Porcine Serum Protein for Fe-chelating Peptides by Response Surface Methodology |
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| Feng ZHU,Jun GUO,Cuiling FAN,Mei ZHU,Lin ZHU,Min ZHAO,Xiaomei ZHU,Rui WANG,Hairong LUO,Jianying WANG,Yuxi LI,Wenfeng SONG,Cuiping XIE,Ningning XIE. Optimization of Enzymolysis Process of Porcine Serum Protein for Fe-chelating Peptides by Response Surface Methodology[J]. Agricultural Science ＆ Technology, 2017, 18(7) |
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| Authors: | Feng ZHU Jun GUO Cuiling FAN Mei ZHU Lin ZHU Min ZHAO Xiaomei ZHU Rui WANG Hairong LUO Jianying WANG Yuxi LI Wenfeng SONG Cuiping XIE Ningning XIE |
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| Abstract: | This study was conducted to optimize the hydrolysis of porcine serum protein by response surface methodology (RSM).Based on single-factor tests,3-factor 3-level response surface tests were designed by Box-Behnken design method,and the effects of pH,temperature and hydrolysis duration on the response values were analyzed by RSM method.The optimal enzymolysis conditions were obtained as follows:pH value of 7.7,temperature at 46.3 ℃C and time of 7.4 h,under which the predicted degree of hydrolysis was 35.52％,and the chelating rate was 79.41％.This process could provide new ideas for the development of porcine serum protein. |
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| Keywords: | Response surface methodology Porcine serum protein Hydrolysis Degree of hydrolysis |
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