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Effects of 2, 4-Dichlorophenoxy Acetic Acid and Light on Growth of Gerbera (Gerbera jamesonii cv.Daxueju) Callus
Authors:Esther Mabafokeng MOHLAKOLA  Chunzhen CHENG  Yuling LIN  Rongfang GUO  Kyaw Thu MIN  Yukun CHEN  Zhongxiong LAI
Institution:Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Abstract:This study was conducted to determine effects of 2,4-dichlorophenoxy acetic acid (2,4-D) and light on growth of gerbera (Gerbera jamesonii cv.Daxueju) callus.Callus was induced from both petiole and leaf explants of gerbera on Murashige and Skoog medium supplemented with 3% sucrose and various concentrations of 2,4-D and placed under light and dark.Callus induction percentage,callus size and callus fresh and dry weights were efficiently higher when using petiole as explant.MS medium supplemented with 1.5 mg/L 2,4-D showed the highest callus induction percentage of 96.70%.Callus induced under light had larger weight mass.It was indicated that 1.5 mg/L 2,4-D and light could promote growth of gerbera callus from petiole explant.
Keywords:Gerbera jamesonii  Callus  Light  Callus growth
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