EMS处理对牛皮杜鹃组培苗叶片再生及耐热性的影响

该试验以长白山野生牛皮杜鹃组培苗为材料,探讨了EMS处理对牛皮杜鹃耐热性的影响和可能的耐热机理,为牛皮杜鹃推广应用及种质资源的创新奠定基础.结果表明,EMS浓度为0.1％处理2d时,叶片存活率48.92％.接近半致死剂量,可以将其作为耐热性突变体的筛选条件.随着温度的升高,诱变组植株叶绿素、MDA、Pro、SOD、POD活性、可溶性糖、MDA含量与对照均存在显著差异;相同温度时,诱变组植株叶绿素含量、可溶性糖含量、Pro,SOD和POD活性显著高于对照组.MDA含量显著低于对照组.EMS处理在一定程度上提高了牛皮杜鹃组培苗的的耐热性.

Effects of EMS Treatment on Leaf Regeneration and Heat Tolerance of Tissue-cultured Rhododendron chrysanthum Pall.Seedlings

With tissue cultured-seedlings of Rhododendron chrysanthum Pall.in Changbai Mountain as test material,the effect of EMS treatment on the heat tolerance of R.chrysanthum,as well as the possible heat tolerance mechanism,was investigated in this study so as to lay a foundation for the popularization,application and germplasm resource innovation of R.chrysanthum.The results showed that when the leaves of the tissue cultured seedlings were treated with medium with 0.1％ of EMS for 2 d,the survival rate of the leaves was 48.92％,thus the concentration of EMS was close to the semi-lethal dose,and the EMS concentration and treatment time could be used as screening conditions for heat-tolerant mutants.The plantlets regenerated from EMS-treated leaves were treated with different temperatures,and related physiological indicators were determined.The results showed that the chlorophyll content,MDA content,Pro content,SOD activity,POD activity and soluble sugar content of regeneration plantlets in the treatment group were significantly different from those in the control group.At the same temperature,the chlorophyll content,soluble sugar content,Pro content,SOD activity and POD activity were significantly higher and the MDA content was significantly lower in the treatment group compared with the control.In conclusion,EMS treatment improved the heat tolerance of tissue-cultured R.chrysanthum seedlings.