微波辅助提取竹荪中膳食纤维的工艺优化

以竹荪为原料,用微波辅助法提取其膳食纤维。在单因素试验结果的基础上,采用响应面法对提取工艺进行优化,以微波功率、微波提取温度、提取时间、液料比为自变量,利用Box-Behnken的中心组合设计原理进行响应面设计,优化提取工艺参数。结果表明:竹荪水溶性纤维(SDF)的最佳提取工艺条件为微波功率490 W、微波提取温度60℃、提取时间10 min、液料比20∶1,此条件下竹荪SDF得率可达12.26%,非水溶性纤维(IDF)得率为74.34%,该SDF得率与SDF得率的最大估计值比较,相对误差约为0.65%,且重复性也很好,因此,该优化提取工艺参数准确可靠。

Optimization for Microwave-assisted Extraction of Dietary Fiber from Dictyophora

Dictyophora was used as raw material for extraction of dietary fiber with microwave-assisted method.Response surface methodology(RSM) was used to optimize the extraction process based on single factor tests.Box-Behnken central composite design principles were used for response surface design,with the power of microwave,liquid-to-solid ratio,microwave-assisted extraction time and temperature as four parameters.The optimum extraction conditions were as follows:microwave power of 490 W,extraction temperature of 60 ℃,microwave-assisted extraction time of 10 min,and liquid-to-solid ratio of 20:1.Under the above optimum extraction conditions,the actual yield of SDF from Dictyophora was 12.26％,and the yield of IDF was 74.34％.The relative standard deviation between the theoretical and practical yields was 0.65％,and the results demonstrated a good reproducibility,indicating the effectiveness and reliability of the RSM model.