不同处理方法对野生百脉根(Lotus corniculatus L.)硬实种子发芽率的影响

百脉根是一种重要的豆科牧草,富含营养物质,适应酸性、干旱及贫瘠的土壤,然而种皮的不透性限制种子吸涨,导致种子萌发率低,出苗不整齐.为此,本试验研究了百脉根种子休眠的机理,以及打破种子休眠的适宜方法.试验结果表明,采自青藏高原的百脉根种子的硬实率高达92.3％,以种皮透水性差为特点.擦破种皮、98％浓硫酸处理、冷藏及超低温冷冻后赤霉素浸泡均能有效地打破种子的硬实率,提高发芽率.

Effects of Different Treatment Methods on Germination Rate of Hard Seeds of Wild Lotus Corniculatus L

Birdsfoot trefoil (Lotus comiculatus L.) is high in nutritional value and adapted to slightly acidic,droughty,infertile,or wet soils.However,the impermeable seed coat restricts water imbibition and results in poor seed germination and subsequent stand establishment.The aim of this study was to identify the dormancy mechanism of Birdsfoot trefoil seeds,and investigate the suitable pretreatment to overcome the poor seed germination.Germination behavior was characterized by using the final germination percentage and the mean germination time.The results indicated that coat thickness of dormant seeds was significant larger.The average hard seed rate was up to 92.3％.It was found that mechanical scarification,chemical scarification with concentrated sulfuric acid (98％ H2SO4),and gibberellic acid soaking following freezing below zero temperature were effective in reducing hardseededness and lead to increases of seed germination.