| 牛精子不同获能方法对体外受精效果的影响 |
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| 引用本文： | 佟桂芝,韩永胜,宋斌,王洪宝. 牛精子不同获能方法对体外受精效果的影响[J]. 农业科学与技术, 2017, 18(5) |
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| 作者姓名： | 佟桂芝 韩永胜 宋斌 王洪宝 |
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| 作者单位： | 黑龙江省畜牧研究所,黑龙江齐齐哈尔,161005 |
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| 基金项目： | 现代农业肉牛牦牛产业技术体系专项经费，齐齐哈尔农业攻关项目(NYGG-201524).Supported by Earmarked Fund for China Agriculture (Beef and Yak) Research System，Key Agriculture Research and Development Program of Qiqihar |
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| 摘 要： | 为了提高牛体外受精率,探讨牛冷冻精液不同获能方法对体外受精效果的影响,对牛冷冻解冻精液进行了两次离心获能法与一次离心加上游法获能对体外受精效果影响试验.结果表明,两次离心组受精率卵裂率(75.6-±4.5)％与一次离心加上游法组受精卵裂率(76.4±1.9)％无显著差异(P＞0.05);二次离心组囊胚率(35.7±4.1)％与一次离心加上游法组(36.3±2.7)％差异不显著(P＞0.05).而两次离心组却可以大大节省了体外获能时间.
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| 关 键 词： | 获能方法 离心法 上浮法 屠体卵巢 卵裂率 囊胚率 |
Effects of Different Bovine Sperm Capacitation Methods on Fertilization in vitro |
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| Guizhi TONG,Yongsheng HAN,Bin SONG,Hongbao WANG. Effects of Different Bovine Sperm Capacitation Methods on Fertilization in vitro[J]. Agricultural Science ＆ Technology, 2017, 18(5) |
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| Authors: | Guizhi TONG Yongsheng HAN Bin SONG Hongbao WANG |
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| Abstract: | In order to improve the in vitro fertilization rate of bovine,the effects of different sperm capacitation methods on fertilization were investigated.Total two treatments were designed:two-time centrifugation (C),and one-time centrifugation and swim-up method (CS).The results showed that the cleavage rate in the C treatment group was (75.6±4.5)％,which showed no difference compared with that ((76.4±1.9)％) in the CS treatment group (P＞0.05);there was also no significant difference in blastocyst rate between the two treatment groups ((35.7±4.1)％ vs.(36.3±2.7)％,P＞0.05).However,the CS treatment significantly saved the capacitation time in vitro. |
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| Keywords: | Capacitation method Centrifugation Swim-up method Ovary of carcass Cleavage rate Blastocyst rate |
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