SSR标记技术鉴定新食葵6号品种纯度的研究

[目的]通过利用SSR分子标记技术测定向日葵种子纯度,以期为生产加工应用提供准确、便捷的杂交种种子纯度鉴定方法提供依据.[方法]该研究以新食葵6号及其双亲的DNA为模板,通过DNA提取、PCR扩增反应和 制作电泳的步骤,进行了约100对SSR物分子标记的筛选.[结果]SSR多态引物标记532在新食葵6号母本分别产生特异条带大小为496bp,父本特异标记条带大小为451 bp;引物标记574在母本上特异条带大小为364 bp,父本上的特异标记条带大小为384 bp,同时室内分子鉴定的纯度和田间鉴定纯度基本一致;通过SSR分子标记方法可出可得到区分父本、母本和杂交种的引物标记532和574,这2个引物标记可有效用于鉴定上述新食葵6号杂交种种子的纯度,辨别种子的真伪.[结论]该方法具有简单,快速、准确、重演性高的优点,目前该方法已成为向日葵品种鉴定的主要方法.

Purity Identification of Xinshikui 6 Using SSR Marker Technique

[Objective] SSR molecular marker technique was used to determine the purity of sunflower seed with the aim to provide accurate,convenient method for the identification of the purity of hybrid seeds in production and processing.[Method] With the DNA of Xinshikui 6 and its parents as template,about 100 pairs of SSR molecular markers were screened after DNA extraction,PCR amplification and electrophoresis production.[Results] SSR polymorphic primer marker 532 produced a specific band of 469 bp in the female parent,and a specific band of 451 bp in the male parent;primer marker 574 produced a specific band of 364 bp in the female parent,and a specific band of 384 bp in the male parent.The indoor molecular purity identification and field purity identification were consistent with each other.The primer marker 532 and 574 could be obtained from the SSR molecular marker method to distinguish the male parent,female parent and hybrid of Xinshikui 6,and both of the 2 primer markers can effectively identify the purities of the hybrid seeds of Xinshikui 6,as well as the authenticity of the seeds.[Conclusion] The proposed method was simple,fast,accurate to operate with the advantages of high reproducibility,and it had become the major method in the identification of sunflower varieties.