慈姑中秋水仙碱的提取及抗炎作用研究

[目的]研究慈姑中秋水仙碱的最佳提取工艺及抗炎作用.[方法]考察乙醇浓度、提取时间、提取温度和料液比对慈姑中秋水仙碱提取率的影响,在单因素试验的基础上,采用L9(34)正交试验优化提取工艺参数.根据最佳提取工艺,采用高效液相色谱法,测定慈姑中秋水仙碱的含量.通过体外抗炎试验,来评价慈姑中秋水仙碱的抗炎能力.[结果]慈姑中秋水仙碱的最佳提取工艺条件为:乙醇浓度60％,提取温度50℃,提取时间30 min,料液比1:25 (g/ml).慈姑中秋水仙碱的含量为40.58 μ.g/100 mg.与模型对照组比较,慈姑中秋水仙碱(9.0,4.5g/kg)抑制胸腔液PGE2,TNF-α,IL-1β含量升高(P＜0.05).慈姑中秋水仙碱高剂量组能抑制肺组织TNF-α.IL-1β,MDA含量升高(P＜0.01),中剂量组抑制肺组织IL-1β含量升高(P＜0.01).[结论]慈姑中秋水仙碱具有良好的抗炎作用,抗炎机制可能与影响炎症介质产生和抗氧化作用有关.

Extraction and Anti-inflammatory Effects of Colchicine from Sagittaria sagittifolia

[Objective] This study was to study the optimal extraction technology and anti-inflammatory effects of colchicine from Sagittaria sagittifolia.[Method] The effects of ethanol concentration,extraction time,extraction temperature and solid-liquid ratio on the extraction rate of colchicine from S.sagittifolia were investigated.On the basis of single-factor experiments,an L9 (34) orthogonal test was carried out to optimize the extraction process.According to the optimal extraction process,the content of colchicine in S.sagittifolia was determined by high performance liquid chromatography.The anti-inflammatory ability of colchicine was evaluated through an anti-inflammatory test in vitro.[Result] The optimal extraction process of colchicine from S.sagittifolia was as follows:ethanol concentration of 60％,extraction temperature of 50 ℃,extraction time of 30 min,and solid-liquid ratio of 1:25 (g/ml).The content of colchicine in S.sagittifolia was determined as 40.58 μg/100 mg.Compared with the control,the colchicine from S.sagittifolia (9.0 and 4.5 g/kg) inhibited the increase in PGE2,TNF-α and IL-1β contents in pleural fluid (P＜0.05).High-dose colchicine inhibited the increase in TNF-α,IL-1β and MDA contents in lung tissue (,P＜0.01),and middle-dose colchicine inhibited the increase in IL-1β content in lung tissue (P＜0.01).[Conclusion] The colchicine in S.sagittifolia has a good anti-inflammatory effect,which may be achieved through hindering the production of inflammatory mediators and antioxidation.