| 1株PRRSV类NADC30毒株的分离鉴定及序列分析 |
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| 引用本文： | 崔丹丹,王傲杰,王新港,周峰,彭志锋,王川庆.1株PRRSV类NADC30毒株的分离鉴定及序列分析[J].河南农业科学,2017,46(9). |
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| 作者姓名： | 崔丹丹 王傲杰 王新港 周峰 彭志锋 王川庆 |
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| 作者单位： | 河南农业大学 牧医工程学院,河南 郑州,450002 |
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| 基金项目： | 河南省高校科技创新团队与支持计划资助项目 |
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| 摘 要： | 为了解猪繁殖与呼吸综合征病毒(PRRSV)流行株的变异情况,将PRRSV阳性病料处理后接种Marc-145细胞,成功分离到1株PRRSV毒株,将其命名为HENZXC-4,并对其全基因组序列进行测定和分析。结果表明,HENZXC-4为未发生重组的类NADC30毒株,其NSP2蛋白存在131个氨基酸不连续缺失,且其GP5蛋白存在1个氨基酸插入。
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 分离鉴定 类NADC30株 序列分析 |
Isolation,Identification and Sequence Analysis of a NADC30-like Strain of PRRSV |
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| CUI Dandan,WANG Aojie,WANG Xingang,ZHOU Feng,PENG Zhifeng,WANG Chuanqing.Isolation,Identification and Sequence Analysis of a NADC30-like Strain of PRRSV[J].Journal of Henan Agricultural Sciences,2017,46(9). |
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| Authors: | CUI Dandan WANG Aojie WANG Xingang ZHOU Feng PENG Zhifeng WANG Chuanqing |
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| Abstract: | To investigate the molecular variation characteristics of porcine reproductive and respiratory syndrome virus(PRRSV) in the field,a PRRSV isolate(HENXC-4) was isolated by inoculating the PRRSV positive samples into the Marc-145 cell layers.The whole genome was then sequenced and analyzed.The sequence analysis showed that HENXC-4 was NADC30-like PRRSV without any recombination phenomenon characterized by a discontinuous 131-amino-acid deletion in the NSP2 region.More importantly,it had a 1-amino-acid insertion in GP5,which was absent in other NADC30-like PRRSV strains. |
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| Keywords: | PRRSV isolation and identification NADC30-like strain sequence analysis |
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