甘薯线虫病品种抗性的PCR检测

根据甜菜抗线虫病基因序列,人工合成了三条上游引物和三条下游引物,从中筛选出一对引物,对11份经抗线虫病常规鉴定的甘薯品种(包括6份高抗,2份抗病,3份感病)进行了PCR检测.结果表明:6份高抗品种和1份抗病品种出现了一条630 bp左右大小的特异片段,而3份感病品种未出现任何条带.这与常规鉴定的结果基本吻合,证明分子检测的可行

PCR Detection of Nematode Resistance in Sweet Potato

According to the sequence of nematode resistant gene in sugar beet, three forward primers and three reverse primers were produced by synthetic. A pair of primers were selected among them. 11 sweet potato cultivars (including 6 nematode high resistant, 2 resistant and 3 sensitive cultivars by conventional identification)were detected by PCR. The results showed that a special fragment of about 630 bp appeared in 6 high resistant and 1 resistant cultivars, and nothing in 3 infected cultivars. The result was in correspondence with that of conventional identification, and suggested the feasibility of PCR detection.