大豆磷高效基因GmPHR1和GmPAP4共转化及新种质创制

磷对大豆生长发育至关重要,土壤有效磷缺乏严重影响其产量和品质。目前已有学者通过转基因手段将磷高效相关基因转入大豆,并获得转基因新材料,但多数集中于单基因转化,而双基因共转化研究甚少。本研究在前期获得转磷高效相关基因GmPHR1、GmPAP4大豆新材料基础上,利用农杆菌介导与常规杂交技术进行双基因共转化,结果发现,采用这2种方案均可实现双基因共转化,获得了经PCR及DNA测序分析验证正确的转GmPHR1与GmPAP4双基因新材料“JD12-PHR1-PAP4”。

Transformation of Gm PHR1 and Gm PAP4 Related to High Phosphorus Efficiency and Elite Germplasm Enhancement in Soybean(Glycine max)

Phosphorus is essential for soybean growth and development, and lack of phosphorus in soil has become an important limiting factor for high yield and quality in soybean. Most previous studies focus on transformation of single-gene related to phosphorus efficiency, while the transformation of double-genes into a plant was rarely reported in soybean. In this study, we used transgenic soybean plants with GmPAP4 or GmPHR1 to achieve new transgenic germplasms with double gene (GmPAP4 and GmPHR1) in a plant via Agrobacterium-mediated transformation and conventional hybridization techniques. It was found that both the methods could achieve co-transformation double-genes plants in variety JD12, and the positive plants were verified further by PCR and DNA sequencing methods. Thus, the new transgenic germplasms with GmPHR1 and GmPAP4 named “JD12-PHR1-PAP4” was obtained.