普通小麦春化基因VRN3的表达分析及过表达载体构建

通过实时荧光定量PCR（qRT-PCR）分析了不同发育特性的普通小麦品种VRN3基因的表达,结果表明：VRN3基因在春性品种新春2号叶片中表达呈上升趋势,在雌雄蕊分化期达到最大值,在强冬性品种京841中表达量很低;VRN3在新春2号和京841茎尖中的表达量均极低。以京841的cDNA为模板扩增VRN3基因全长,连接到pCAMBIA3301载体上,构建VRN3基因过表达载体,通过农杆菌茎尖转化法获得了转基因植株,研究结果为春化基因VRN3对小麦春化发育调控的进一步研究提供依据。

Expression Analysis and Construction of Over-Expression Vector of Vernalization Gene VRN3 in Common Wheat

VRN3 expression patterns were analyzed in wheat varieties with different developmental characteristics by qRT-PCR. The results showed that the expression of VRN3 in spring wheat variety Xinchun 2 increased gradually and reached the highest level in pistil and stamen differentiation stage; VRN3 expression level in winter wheat variety Jing 841 was very lower, also in the shoot meristems of both wheat varieties. Using Jing841 cDNA as template and pCAMBIA3301 as expression vector, we amplified the full length of VRN3 gene, constructed VRN3 over-expression vector and transferred into wheat by agro-bacterium mediated shoot apices and obtained transgenic plants by PCR identification. The results provide the basis for further analyzing the function of VRN3 on wheat vernalization regulation.