| 玉米赤霉烯酮降解酶基因（ZEN-jjm）的克隆、表达及活性分析 |
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| 引用本文： | 程波财,史文婷,罗洁,彭福中,万翠香,魏华. 玉米赤霉烯酮降解酶基因（ZEN-jjm）的克隆、表达及活性分析[J]. 农业生物技术学报, 2010, 18(2): 225-230. DOI: 10.3969/j.issn.1674-7968.2010.02.004 |
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| 作者姓名： | 程波财 史文婷 罗洁 彭福中 万翠香 魏华 |
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| 作者单位： | 1. 中南大学资源加工与生物工程学院,长沙,410083;食品科学与技术国家重点实验室,南昌大学,南昌,330047
2. 食品科学与技术国家重点实验室,南昌大学,南昌,330047 |
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| 基金项目： | 国家自然科学基金，江西省科技支撑计划项目(益生菌降解谷物和饲料中真菌毒素的应用研究)资助 |
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| 摘 要： | 玉米赤霉烯酮(zearalenone,ZEN)是世界上污染范围最广泛的一种镰刀霉毒素,能导致人或动物不孕、流产等症状,损害内脏器官并促进癌细胞的生长.本文通过设计1对特异性引物,从粉红螺旋聚孢霉(Gliocladium roseum)中克隆获得大小为795 bp的DNA片段(ZEN-jjm),通过构建E. coil原核表达载体进行表达.经HPLC检测证实,IPTG诱导后的细胞裂解上清能在3 h内完全降解液体中1μg/mL的ZEN.序列分析结果表明ZEN-jjm与zhd101存在9个碱基的差异,ZEN-jjm编码的ZEN降解酶与文献报道的乳糖水解酶存在3个氨基酸的差异,但ZEN毒素降解实验证实二者活性相似.
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| 关 键 词： | 玉米赤霉烯酮 粉红螺旋聚孢霉 ZEN降解酶 基因克隆 表达 |
Cloning of Zearalenone-degraded Enzyme Gene(ZEN-jjm) and its Expression and Activity Analysis |
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| Cheng Bocai,Shi Wenting,Luo Jie,Peng Fuzhong,Wan Cuixiang,Wei Hua. Cloning of Zearalenone-degraded Enzyme Gene(ZEN-jjm) and its Expression and Activity Analysis[J]. Journal of Agricultural Biotechnology, 2010, 18(2): 225-230. DOI: 10.3969/j.issn.1674-7968.2010.02.004 |
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| Authors: | Cheng Bocai Shi Wenting Luo Jie Peng Fuzhong Wan Cuixiang Wei Hua |
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| Affiliation: | Cheng Bocai 1,2 Shi Wenting 2 Luo Jie 2 Peng Fuzhong 2 Wan Cuixiang 2 Wei Hua 2 1 School of Minerals Processing & Bioengineering,Centre South University,Changsha,410083,2 State Key Laboratory of Food Science , Technology,Nanchang University,Nanchang,330047 |
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| Abstract: | Zearalenone(ZEN) is the most widely distributed Fusarium toxins all over the world.ZEN can lead to a series of estrogenic effects symptoms such as infertility and abortion, damage the internal organs, and even promote the cancer cells to grow.One 795 bp DNA fragment(ZEN-jjm ) from Gliocladium roseum in charge of lactonohydrolase was cloned into a vector and transferred into E.coli, the degradation capabilitites of expressed protein in the supernatant of disrupted cells was verified by HPLC test.In 3 h the c... |
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| Keywords: | Zearalenone Gliocladium roseum ZEN-JJM Gene cloning Expression |
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