| 刺槐Genomic-SSR与EST-SSR遗传差异性分析 |
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| 引用本文： | 董黎,张江涛,文彦忠,牛东升,田书勇,孙宇涵,李云. 刺槐Genomic-SSR与EST-SSR遗传差异性分析[J]. 中国农学通报, 2019, 35(19): 49-57. DOI: 10.11924/j.issn.1000-6850.casb18030010 |
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| 作者姓名： | 董黎 张江涛 文彦忠 牛东升 田书勇 孙宇涵 李云 |
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| 作者单位： | 1.林木育种国家工程实验室 北京林业大学生物科学与技术学院;2.河南省林业科学研究院;3.吉县林木良种繁育场;4.冠县国有苗圃 |
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| 基金项目： | 北京林业大学重大科研成果培育项目“刺槐种质资源评价、品种选育与产业化应用”(2017CGP007);林业公益性行业科研专项“刺槐属种 质资源收集保存与创新利用研究”(201304116);国家林业局科技发展中心项目“北林槐1 号新品种转化应用试点”(XPC-2015-3);中央高校基本科研 业务费专项资金资助“重要生态抗逆树种育种与高效繁殖技术”(2016BLPX13);国家林业局科技推广项目“四倍体刺槐扦插繁殖方法推广示范” ([2015]53 号);国家自然科学基金项目“刺槐不去雄条件下实施控制授粉的花粉竞争与子代选择机制”(31570677)。 |
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| 摘 要： | [目的]对刺槐Genomic-SSR与EST-SSR的遗传差异性进行研究,为今后刺槐遗传多样性分析等育种相关研究中合理选用不同来源的SSR分子标记奠定基础。[方法]选取来自美国四个不同采集地的种子育出的12个刺槐个体,试剂盒提取DNA后分别利用9对Genomic-SSR引物和9对EST-SSR引物进行扩增,并采取毛细管电泳检测扩增产物。利用所得条带信息及相关软件对两种SSR分子标记进行多态性、遗传相似系数相关性以及聚类等方面的比较分析。[结果]刺槐Genomic-SSR平均检测到的条带数为6.0、Shannon多样性指数为1.3833、观测杂合度为0.5749、期望杂合度为0.6832;EST-SSR平均检测到的条带数为5.1、Shannon多样性指数为1.2711、观测杂合度为0.5648、期望杂合度为0.6526。由Genomic-SSR计算得到的个体间的遗传相似系数以及聚类结果与两种SSR标记综合计算得到的遗传相似系数和聚类结果更为相似。[结论]刺槐Genomic-SSR与EST-SSR存在一定的遗传差异性,但差异并不显著;刺槐Genomic-SSR能更加准确地揭示基因型之间的遗传关系;刺槐EST-SSR具有相对较强的保守性。
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| 关 键 词： | 甘蓝型油菜 甘蓝型油菜 生态区 遗传效应 杂种优势 |
| 收稿时间： | 2018-03-03 |
Genetic differences revealed by Genomic-SSR and EST-SSRin black locust (Robinia pseudoacacia) |
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| Abstract: | [Objective] The genetic differences of Genomic-SSR and EST-SSR in black locust was studied in order to lay the foundation of rational use of different SSR molecular markers in genetic diversity of black locust and other related research. [Method] 12 individuals of black locust propagated by seeds from four collection sites in the USA was selected. Total genomic DNA was extracted by kit. 9 Genomic-SSR and 9 EST-SSR primers were used for PCR amplification. The products were separated by capillary electrophoresis. Comparative analysis of polymorphism, correlation of genetic similarity coefficients and cluster between Genomic-SSR and EST-SSR was conduct by related software according to the information of the bands. [Result] The average number of bands detected by Genomic-SSR was 6.0, shannon index 1.3833, observed heterozygosity 0.5749 and expected heterozygosity 0.6832. The average number of bands detected by EST-SSR was 5.1, Shannon index 1.2711, observed heterozygosity 0.5648 and expected heterozygosity 0.6526. Genetic similarity coefficients and cluster result by Genomic-SSR were more similar to Genomic-SSR + EST-SSR. [Conclusion] There are genetic differences between Genomic-SSR and EST-SSR of black locust, but the differences are not significant. Genomic-SSR of black locust can more accurately reflect genetic relationship of different genotypes. EST-SSR of black locust is more conservative. |
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