‘蓝塔’圆柏茎段组织石蜡切片技术研究

【研究目的】为了得到‘蓝塔’(Sabina chinesis ‘Lanta’)圆柏茎段横切面的石蜡切片制作方法，从而为进一步研究其扦插生根解剖学发生机理奠定基础。【方法】选择‘蓝塔’圆柏当年生16 cm长茎段，剪取基部5 mm为材料，对传统石蜡制片法软化、粘片、染色等步骤进行改良。【结果】结果表明：20%盐酸浸泡24 h和水煮4天优于浓盐酸浸泡24 h、水煮10天、甘油-乙醇浸泡10天这3种软化方案；合理的脱水、透明时间为每级2 h ；最优切片厚度为10 μm；采用明胶粘片剂与40%乙醇的混合液较传统粘片方法不易脱片；番红染色60 min、固绿10 s得到的切片染色清晰且省时间。【结论】该研究为‘蓝塔’圆柏茎段切片技术提供了一套具体的操作步骤，同时也为与‘蓝塔’圆柏茎段结构相似树种的研究提供技术参考。

Stem Segment Tissue of Sabina chinesis‘Lanta’: Paraffin Section Technology

The paper aims to obtain the paraffin section preparation method for the cross section of the Sabina chinesis‘Lanta’stem, and lay foundation for further study on the mechanism of rooting anatomy of the cuttage. We selected 16 cm long stem of Sabina chinesis‘Lanta’, cut the base 5 mm as the material, and modified the traditional paraffin section, such as softening, sticking and dyeing. The results showed that: the softening schemes of 20% hydrochloric acid immersed for 24 h and boiled for 4 d were better than that of concentrated hydrochloric acid immersed for 24 h, boiled for 10 d and glycerol- ethanol soaked for 10 d; reasonable dehydration and transparent time was 2 h per level; the optimal paraffin section thickness was 10 μm; the traditional adhesive method was modified with the mixture of gelatin tablets and 40% ethanol; paraffin sections obtained by staining with safranin for 60 min and fast green staining for 10 s were clear and time saving. This study provides a set of specific operation steps for the paraffin section technique of Sabina chinesis‘Lanta’ stem, and also provides a reference for the study on similar tree species.