水分胁迫对春大豆叶片保护酶活性及相对电导率的影响

为了研究了水分胁迫对大豆叶片保护酶活性和相对含水率的影响，以3 个大豆品种‘黑农57’、‘改良168’、‘绥农26’为材料，采用盆栽控水试验，设置中度干旱、重度干旱和适宜水分处理。结果显示：（1）在适宜水分条件下，叶片超氧化物歧化酶(SOD)和过氧化物酶(POD)活性表现为先增加后降低的趋势，过氧化氢酶(CAT)活性和相对电导率表现为降低的趋势。（2）各时期SOD和CAT活性为中度胁迫>重度胁迫>适宜水分，差异显著；各时期POD活性和苗期-盛花期的相对电导率为重度胁迫>中度胁迫>适宜水分，差异显著。（3）在干旱胁迫条件下，POD活性和SOD活性，‘黑农57’>‘改良168’>‘绥农26’；CAT活性品种间差异不显著。综上，在干旱胁迫下，春大豆叶片保护酶活性和相对电导率比在适宜水分下活性要高，体现了春大豆对干旱环境的自我调节能力。

Water Stress Affects Protective Enzyme Activity and Relative Conductivity of Spring Soybean

To study the effects of water stress on the protective enzymes activity and relative water content of soybean leaves, three soybean varieties,‘Heinong 57’,‘Improved 168’and‘Suinong 26’, are used in pot water control experiment. Moderate drought, severe drought and suitable moisture treatment are set up. The results showed that: (1) the activity of superoxide dismutase and peroxidase increased first and then decreased under suitable water condition, and the catalase activity and relative conductivity decreased; (2) the activities of superoxide dismutase and catalase were as follows: moderate drought>severe drought>suitable moisture, and the differences were significant, the peroxidase activity in each period and relative conductivity at seedlingflowering stage were as follows: severe stress > moderate stress > suitable water, and the differences were also obvious. (3) Under drought stress, the activities of peroxidase and superoxide dismutase were as follows: ‘Heinong 57’>‘Improved 168’>‘Suinong 26’, the catalase activity among varieties had no significant difference. In conclusion, under drought stress, the activity of protective enzymes and relative conductivity inspring soybean leaves are higher than that under suitable water condition, reflecting the self-regulation ability of spring soybean to drought environment.