蘘荷离体快速繁殖技术研究

为实现蘘荷［Ｚｉｎｇｉｂｅｒｍｉｏｇａ（Ｔｈｕｎｂｅｒｇ）Ｒｏｓｃｏｅ］的离体快速繁殖，提高繁殖率，缩短培养周期，本试验以蘘荷成熟种子萌发出的幼苗作为外植体，以ＭｕｒａｓｈｉｇｅａｎｄＳｋｏｏｇ（ＭＳ）为基本培养基，添加不同浓度的６-苄基腺嘌呤（６-Ｂｅｎｚｙｌａｍｉｎｏｐｕｒｉｎｅ，６-ＢＡ）、激动素（Ｋｉｎｅｔｉｎ，ＫＴ）、噻苯隆（Ｔｈｉｄｉａｚｕｒｏｎ，ＴＤＺ）和萘乙酸（１-Ｎａｐｈｔｈｙｌａｃｅｔｉｃ ａｃｉｄ，ＮＡＡ），探究这些植物生长调节剂对蘘荷芽增殖倍数及一步成苗的影响．结果表明，低浓度ＴＤＺ对蘘荷丛生芽增殖影响显著，以添加０.０１ｍｇ／ＬＴＤＺ的增殖效果最优，丛生芽增殖倍数为９.０；６-ＢＡ次之，在添加２.０ｍｇ／Ｌ６-ＢＡ时，丛生芽增殖倍数最高，为７.２８；ＫＴ效果最差，在添加３.０ｍｇ／ＬＫＴ时，丛生芽增殖倍数最高时仅为４５．然而，在丛生芽增殖倍数高的ＭＳ＋０.０１ｍｇ／ＬＴＤＺ培养基上，其生根率（３８％）、平均株高（２.２８ｃｍ）、单株平均根数（０.８０）及平均根长（０.５２ｃｍ）均较低，所获得的再生植株不适宜移栽．而在添加ＭＳ＋２.０ｍｇ／Ｌ６-ＢＡ的增殖培养基上，再生植株平均株高５.２０ｃｍ、生根率１００％、单株平均根数９.２０，平均根长４.０８ｃｍ，株形好，不经生根培养即可进行移栽．通过本研究得到满足较高增殖率且同时生根后适宜移栽的一步成苗培养基为ＭＳ＋３.０～４.０ｍｇ／Ｌ６-ＢＡ＋０.０１ｍｇ／ＬＮＡＡ及ＭＳ＋２.０～４.０ｍｇ／Ｌ６-ＢＡ．

In vitro rapid propagation of Zingiber mioga (Thunberg) Roscoe

The seedlings germinated from mature seeds of Zingiber mioga(Thunberg) Roscoe were used as explants and the Murashige and Skoog(MS) was employed as base medium supplemented with different concentration of 6-Benzylaminopurine(6-BA), Kinetin(KT), Thidiazuron(TDZ), and 1-naphthylacetic acid(NAA) to study the effects of these plant growth regulators(PGRs) on shoot proliferation times and one-step seedling formation. The result showed that low concentration TDZ(0.01 mg/L) had best effect on shoot multiplication, the shoot proliferation rate was 9.0. The most suitable 6-BA concentration was 2.0 mg/L, the shoot proliferation rate was 7.28. KT had the worst multiplication effect, and the highest proliferation rate was 4.50 on MS+3.0 mg/L KT. However, although MS+0.01 mg/l TDZ are best for shoot multiplication, low rooting rate(38%), mean shoot length(2.28 cm), mean rooting number(0.80) and mean root length(0.52 cm) were obtained in this medium, the regenerated seedlings from the medium failed to meet requirement of acclimatization. MS+2.0 mg/L 6-BA exhibited good growth condition among all growing indexes such as mean shoot length(5.20 cm), rooting rate(100%), mean root number(9.20) and mean root length(4.08 cm). MS+2.0-4.0 mg/L 6-BA and MS+3.0-4.0 mg/L 6-BA+0.01 mg/L NAA were suitable for one-step seedling formation.