| 人体醛缩酶C cDNA的克隆及其在家蚕体内的表达(英文) |
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| 引用本文： | 段家龙,佐藤秀对,原三郎. 人体醛缩酶C cDNA的克隆及其在家蚕体内的表达(英文)[J]. 蚕业科学, 2005, 31(4): 449-453 |
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| 作者姓名： | 段家龙 佐藤秀对 原三郎 |
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| 作者单位： | 1. 安徽农业大学,合肥,230036
2. 日本京都工艺纤维大学,京都,606-8585 |
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| 摘 要： | 利用人体醛缩酶C cNDA的一对特异性引物A ld-5′(5′-ggatcccctcactcgtaccca-3′)和A ld-3′(5′g-gatcctcag-taggcatggtt-3′),从人脑cDNA文库中PCR扩增出人体醛缩酶C cDNA,并依次被克隆进克隆载体pCR2.1、转移载体pAcGP67B以及来源于AcNPV和BmNPV的杂交重组病毒HyNPV。通过对家蚕血液的SDS-PAGE电泳和酶活力测定,证明人体醛缩酶C cDNA在家蚕体内成功得到表达。
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| 关 键 词： | 人体醛缩酶C 基因表达 杂交重组病毒 家蚕 |
| 文章编号： | 0257-4799(2005)04-0449-05 |
| 修稿时间： | 2005-06-03 |
Cloning and Expression of Human FBP Aldolase C cDNA in Silkworm Bombyx mori |
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| DUAN Jialong,Sato Hideki,Saburo Hara. Cloning and Expression of Human FBP Aldolase C cDNA in Silkworm Bombyx mori[J]. Acta Sericologica Sinica, 2005, 31(4): 449-453 |
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| Authors: | DUAN Jialong Sato Hideki Saburo Hara |
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| Abstract: | Fructose-1,6-bisphosphate (FBP) aldolase C cDNA from human brain cDNA library was amplified via PCR with primers of Aid-5' (5'-ggatcccctcactcgtaccca-3') and Aid-3' (5'-ggatcctcagtaggcatggtt-3')and was successively cloned into cloning vector pCR2.1, transfer vector pAcGP67B, and baculovirus HyNPV (hybrid baculovirus of Autographa californica nuclear polyhedrosis virus and Bombyx mori nuclear polyhedrosis virus). The SDS-PAGE and enzyme activity analyses indicated that recombinant intact human Aldolase C protein was successfully expressed in silkworm larval blood via recombinant virus HyNPV-hAld-c. |
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| Keywords: | Human FBP aldolase C Gene expression HyNPV Bombyx mori |
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