| 糜子感染黑穗病菌后的生理变化及与抗病性关系 |
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| 引用本文： | 张骥如飞,周瑜,杨璞,王鹏科,高金锋,高小丽,冯佰利.糜子感染黑穗病菌后的生理变化及与抗病性关系[J].中国农业大学学报,2015,20(3):108-113. |
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| 作者姓名： | 张骥如飞 周瑜 杨璞 王鹏科 高金锋 高小丽 冯佰利 |
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| 作者单位： | 西北农林科技大学 农学院/旱区作物逆境生物学国家重点实验室,陕西杨凌,712100 |
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| 基金项目： | 国家谷子糜子产业技术体系(CARS-07-12.5-A9); 国家自然科学基金资助项目(31371529); 科技支撑计划(2014BAD07B03); 陕西省科技厅难题攻关项目资助 |
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| 摘 要： | 为明确糜子对黑穗病的抗性生理机制,在大田条件下,以不同抗性水平的10个糜子品种为材料,采用种子饱和接种法人工接种黑粉菌,测定了三叶期不同糜子品种叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、β-1,3-葡聚糖酶、几丁质酶活性以及丙二醛(MDA)含量。结果表明,人工接种黑粉菌后不同抗性水平糜子品种叶片SOD、β-1,3-葡聚糖酶和几丁质酶活性有所提高,最大增幅分别达92%、16%和31%;MDA含量有所增加,最大增幅达20%;POD活性呈不同变化。人工接种条件下抗性水平较高品种的保护酶系统酶活性以及病程相关蛋白活性提升幅度较大,MDA含量增加幅度较小。各生理指标与抗病性的相关分析表明,SOD、POD、β-1,3-葡聚糖酶以及几丁质酶活性与发病率呈极显著负相关,相关系数分别为0.941、0.468、0.514和0.757;MDA含量与发病率呈显著正相关,相关系数为0.378。
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| 关 键 词： | 糜子 黑穗病 保护酶 病程相关蛋白 |
| 收稿时间： | 2014/11/18 0:00:00 |
Study on physiological changes and correlation with resistance level to the head smut of broomcorn millet after an infection with Sphacelotheca destruen |
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| ZHANG Ji-ru-fei,ZHOU Yu,YANG Pu,WANG Peng-ke,GAO Jin-feng,GAO Xiao-li and FENG Bai-li.Study on physiological changes and correlation with resistance level to the head smut of broomcorn millet after an infection with Sphacelotheca destruen[J].Journal of China Agricultural University,2015,20(3):108-113. |
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| Authors: | ZHANG Ji-ru-fei ZHOU Yu YANG Pu WANG Peng-ke GAO Jin-feng GAO Xiao-li and FENG Bai-li |
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| Institution: | College of Agronomy/State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling 712100, China;College of Agronomy/State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling 712100, China;College of Agronomy/State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling 712100, China;College of Agronomy/State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling 712100, China;College of Agronomy/State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling 712100, China;College of Agronomy/State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling 712100, China;College of Agronomy/State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling 712100, China |
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| Abstract: | |
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| Keywords: | broomcorn millet head smut protective enzyme pathogenesis related protein |
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