| 转MdSPDS1-AtCBF1双价基因烟草抗逆境研究 |
| |
| 引用本文： | 赵先海,湛欣,邓小梅,袁金英,赵帅.转MdSPDS1-AtCBF1双价基因烟草抗逆境研究[J].中国农业大学学报,2015,20(3):46-50. |
| |
| 作者姓名： | 赵先海 湛欣 邓小梅 袁金英 赵帅 |
| |
| 作者单位： | 华南农业大学 林学院/广东省森林植物种质创新与利用重点实验室,广州,510642 |
| |
| 基金项目： | 国家林业局林业公益性行业科研专项(201004020) |
| |
| 摘 要： | 为研究由诱导型启动子Atrd29A介导表达的2个抗胁迫基因MdSPDS1和AtCBF1对转化烟草的影响,对转化双价基因烟草进行了抗逆境试验,主要包括:耐盐性试验、耐渗透性试验和耐寒性试验。结果表明:转基因烟草在添加了20μmol/L Al2(SO4)3、20μmol/L CuSO4、100mmol/L NaCl的培养基上生长时,质量和高度的增加率都大于未转基因烟草,表现出一定的抗盐性。同样,在添加了50mmol/L Mannitol、4mmol/L PEG的培养基上,转基因烟草也得到了比未转基因烟草更好的生长状态。对转基因烟草组培苗进行-4℃耐寒性试验,具有比未转基因烟草更低的细胞死亡和超氧阴离子累积。移栽到温室中的转基因烟草在-4℃处理时叶片萎蔫减缓,相对电导率低于未转基因烟草。
|
| 关 键 词： | 烟草 MdSPDS1-ATCBF1 耐盐性 耐渗透性 耐寒性 |
| 收稿时间： | 2014/9/28 0:00:00 |
Stress resistance of the transgenic MdSPDS1-AtCBF1 double genes tobacco plants |
| |
| ZHAO Xian-hai,ZHAN Xin,DENG Xiao-mei,YUAN Jin-ying and ZHAO Shuai.Stress resistance of the transgenic MdSPDS1-AtCBF1 double genes tobacco plants[J].Journal of China Agricultural University,2015,20(3):46-50. |
| |
| Authors: | ZHAO Xian-hai ZHAN Xin DENG Xiao-mei YUAN Jin-ying and ZHAO Shuai |
| |
| Institution: | College of Forestry/ Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, China;College of Forestry/ Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, China;College of Forestry/ Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, China;College of Forestry/ Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, China;College of Forestry/ Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, China |
| |
| Abstract: | |
| |
| Keywords: | tobacco MdSPDS1-ATCBF1 salt tolerance osmotic tolerance cold tolerance |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国农业大学学报》浏览原始摘要信息 |
| 点击此处可从《中国农业大学学报》下载免费的PDF全文 |
|
