| 黄瓜膜联蛋白基因CsANN2的克隆和表达分析 |
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| 引用本文： | 李殿波,王晋芳,石锦,李双桃,向娟,张磊,赵冰,郭仰东. 黄瓜膜联蛋白基因CsANN2的克隆和表达分析[J]. 中国农业大学学报, 2015, 20(3): 82-89 |
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| 作者姓名： | 李殿波 王晋芳 石锦 李双桃 向娟 张磊 赵冰 郭仰东 |
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| 作者单位： | 中国农业大学 农学与生物技术学院/设施蔬菜生长发育调控北京市重点实验室,北京,100193 |
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| 基金项目： | 国家重点基础研究发展计划(2012CB113900); 北京市自然科学基金(6152015) |
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| 摘 要： | 以‘津研四号’黄瓜品种为材料,克隆得到一个黄瓜膜联蛋白基因的cDNA全长,命名为CsANN2。为进一步探究黄瓜CsANN2基因的功能,对CsANN2基因编码的蛋白序列进行生物信息学分析后,发现其理化性质、保守结构域以及多个功能蛋白位点都和其他植物的膜联蛋白一致。系统进化树分析表明,黄瓜CsANN2与AtANN2、AtANN6、AtANN7、BjANN2和BjANN6有较近的亲缘关系。亚细胞定位结果显示,CsANN2蛋白在细胞膜、细胞质与细胞核内都有分布。qRT-PCR结果分析表明,CsANN2基因受ABA、SA、PEG和H2O2诱导表达,但对NaCl胁迫无响应。
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| 关 键 词： | 黄瓜 膜联蛋白 CsANN2 生物信息学 表达特征 |
| 收稿时间： | 2014-11-04 |
Cloning and expression of CsANN2 gene in cucumber |
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| LI Dian-bo,WANG Jin-fang,SHI Jin,LI Shuang-tao,XIANG Juan,ZHANG Lei,ZHAO Bing and GUO Yang-dong. Cloning and expression of CsANN2 gene in cucumber[J]. Journal of China Agricultural University, 2015, 20(3): 82-89 |
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| Authors: | LI Dian-bo WANG Jin-fang SHI Jin LI Shuang-tao XIANG Juan ZHANG Lei ZHAO Bing GUO Yang-dong |
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| Affiliation: | College of Agronomy and Biotechnology/Beijing Key Laboratory of Growth and Developmental Regulation forProtected Vegetable Crops, China Agricultural University, Beijing 100193, China;College of Agronomy and Biotechnology/Beijing Key Laboratory of Growth and Developmental Regulation forProtected Vegetable Crops, China Agricultural University, Beijing 100193, China;College of Agronomy and Biotechnology/Beijing Key Laboratory of Growth and Developmental Regulation forProtected Vegetable Crops, China Agricultural University, Beijing 100193, China;College of Agronomy and Biotechnology/Beijing Key Laboratory of Growth and Developmental Regulation forProtected Vegetable Crops, China Agricultural University, Beijing 100193, China;College of Agronomy and Biotechnology/Beijing Key Laboratory of Growth and Developmental Regulation forProtected Vegetable Crops, China Agricultural University, Beijing 100193, China;College of Agronomy and Biotechnology/Beijing Key Laboratory of Growth and Developmental Regulation forProtected Vegetable Crops, China Agricultural University, Beijing 100193, China;College of Agronomy and Biotechnology/Beijing Key Laboratory of Growth and Developmental Regulation forProtected Vegetable Crops, China Agricultural University, Beijing 100193, China;College of Agronomy and Biotechnology/Beijing Key Laboratory of Growth and Developmental Regulation forProtected Vegetable Crops, China Agricultural University, Beijing 100193, China |
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| Abstract: | |
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| Keywords: | cucumber annexin CsANN2 bioinformatics expression pattern |
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