| 黄瓜绿斑驳花叶病毒在葫芦上的种传规律及热处理效果评价 |
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| 引用本文： | 宋顺华,吴萍,宫国义,孟淑春,耿丽华.黄瓜绿斑驳花叶病毒在葫芦上的种传规律及热处理效果评价[J].植物保护学报,2015,42(5):841-847. |
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| 作者姓名： | 宋顺华 吴萍 宫国义 孟淑春 耿丽华 |
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| 作者单位： | 北京市农林科学院蔬菜研究中心, 农业部华北地区园艺作物生物学与种质创制重点实验室, 农业部都市农业 (北方)重点实验室, 北京 100097;北京京研益农科技发展中心, 北京 100097;北京市农林科学院蔬菜研究中心, 农业部华北地区园艺作物生物学与种质创制重点实验室, 农业部都市农业 (北方)重点实验室, 北京 100097;北京京研益农科技发展中心, 北京 100097;北京市农林科学院蔬菜研究中心, 农业部华北地区园艺作物生物学与种质创制重点实验室, 农业部都市农业 (北方)重点实验室, 北京 100097;北京京研益农科技发展中心, 北京 100097;北京市农林科学院蔬菜研究中心, 农业部华北地区园艺作物生物学与种质创制重点实验室, 农业部都市农业 (北方)重点实验室, 北京 100097;北京市农林科学院蔬菜研究中心, 农业部华北地区园艺作物生物学与种质创制重点实验室, 农业部都市农业 (北方)重点实验室, 北京 100097 |
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| 基金项目： | 国家现代农业产业技术体系北京市西甜瓜创新团队专项,北京市农林科学院创新能力建设专项(KJCX20140111),国家科技支撑计划项目(2011BAD35B07) |
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| 摘 要： | 为明确黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)在葫芦上的种传规律,以西瓜砧木葫芦种子为材料,采用双抗体夹心酶联免疫吸附测定法(DAS-ELISA)、反转录聚合酶链式反应(RT-PCR)与生物学检测相结合的方法研究葫芦种子的带毒率和传毒率的关系,并评价了干热处理对病毒的钝化效果。结果表明,DAS-ELISA灵敏度检测种子时,在感染种子研磨液∶健康种子研磨液为1∶1 000时,带毒量仍能检测出阳性,RT-PCR和DAS-ELISA两种方法均能准确检测葫芦种子的带毒情况;6个批次的葫芦种子有4个批次呈阳性,带毒率在0~100%之间,贮藏1年后的传毒率在0~5.6%之间;4个为CGMMV阳性的种子批经干热处理后,仅1株实生苗呈阳性。研究表明,CGMMV在葫芦作物上的隐性带毒现象非常普遍;种子的带毒率高而传毒率低,以表面带毒为主,且非常稳定;72℃72 h干热处理葫芦种子能有效地钝化CGMMV。
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| 关 键 词： | 黄瓜绿斑驳花叶病 葫芦种子 带毒率 传毒率 干热处理 |
| 收稿时间： | 2014/11/26 0:00:00 |
Seed transmission of Cucumber green mottle mosaic virus on gourd and the evaluation of the effect of heat treatment |
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| Song Shunhu,Wu Ping,Gong Guoyi,Meng Shuchun and Geng Lihua.Seed transmission of Cucumber green mottle mosaic virus on gourd and the evaluation of the effect of heat treatment[J].Acta Phytophylacica Sinica,2015,42(5):841-847. |
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| Authors: | Song Shunhu Wu Ping Gong Guoyi Meng Shuchun and Geng Lihua |
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| Institution: | Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in North China Area, Key Laboratory of Urban Agriculture (North), Ministry of Agriculture; Beijing Vegetable Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China;Beijing Jingyan Yinong Sci-Tech Development Center, Beijing 100097, China;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in North China Area, Key Laboratory of Urban Agriculture (North), Ministry of Agriculture; Beijing Vegetable Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China;Beijing Jingyan Yinong Sci-Tech Development Center, Beijing 100097, China;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in North China Area, Key Laboratory of Urban Agriculture (North), Ministry of Agriculture; Beijing Vegetable Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China;Beijing Jingyan Yinong Sci-Tech Development Center, Beijing 100097, China;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in North China Area, Key Laboratory of Urban Agriculture (North), Ministry of Agriculture; Beijing Vegetable Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in North China Area, Key Laboratory of Urban Agriculture (North), Ministry of Agriculture; Beijing Vegetable Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China |
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| Abstract: | |
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| Keywords: | Cucumber green mottle mosaic virus (CGMMV) gourd seed seed contamination rate seed-to-seedling transmission rate dry heat treatment |
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