牛病毒性腹泻病毒地方株的分离与鉴定

从河南省不同地区规模化肉牛场牛病毒性腹泻 (BVD)疑似病例中采集病料 ,将处理好的 6份病料接种MDBK细胞 ,并盲传 4代 ,得到了 2株可产生细胞病变的病毒。 2株病毒的TCID50 分别为 10 - 5.59和 10 - 5.51;琼脂扩散试验表明 ,2株病毒均能与牛病毒性腹泻病毒 (BVDV)OregonC2 4 标准阳性血清反应 ,出现沉淀线 ;中和试验表明 ,2株病毒均能被BVDV标准阳性血清中和 ,中和指数分别为 10 3.30 和 10 3.16 ;电镜观察到圆形 ,直径为 4 0～ 6 0nm ,有囊膜 ,囊膜表面有突起的病毒粒子 ,与BVDV颗粒基本一致。因此 ,确定分离的 2株病毒均为BVDV ,分别将其命名为HN - 1株和HN - 2株。

Isolation and Identification of BVDV Regional Strains in Henan Province

specimen collected from the doubtful bovine viral diarrhea(BVD) cases in the intensive beef farms of different areas in Henan province were inoculated by MDBK cells. 4 generations were reproduced blindly.Two strains could cause cells pathological changes.Agar gel precipitation(AGP) showed that two strains appeared positive reactions with BVDV Oregon C_ 24;and could be neutralized by BVDV standard positive serum,with the neutralization indices of 10 3.30 and 10 3.16 respectively.TCID_ 50 of the two virus strains were 10 -5.59 and 10 -5.51.Under electronic microscope, round-shaped, virus particles with vesicular membrane could be observed.The diameter vesicular membrane was 40-60 nm,and there are protuberances on the surface of the vesicular membrane,being as the same as BVDV particles.Therefore,it was confirmed that the two isolated strains were BVDV, named HN-1 and HN-2 strain.