| 韭菜花粉管通道法转基因技术 |
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| 引用本文： | 张伟,刘宏敏,杨宛玉,乔保建,张明,王利亚,李延龙. 韭菜花粉管通道法转基因技术[J]. 山东农业科学, 2010, 0(3): 8-10 |
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| 作者姓名： | 张伟 刘宏敏 杨宛玉 乔保建 张明 王利亚 李延龙 |
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| 作者单位： | 平顶山市农业科学院农业生物技术重点实验室,河南 平顶山,467001 |
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| 基金项目： | 河南省重点科技攻关项目“韭菜转基因抗虫育种”资助(编号072102120005) |
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| 摘 要： | 花粉管通道法介导韭菜转基因尚处于研究阶段,它是基因工程技术与常规杂交育种相结合的育种方法。本文详尽介绍了外源dna片段在韭菜基因组中整合的理论基础和受精过程转化的理论基础,韭菜利用花粉管通道法转基因的操作程序、影响因素及优缺点和前景。
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| 关 键 词： | 花粉管通道法 韭菜 转基因育种 |
Leek Pollen-tube Pathway Transgenic technique |
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| ZHANG Wei,LIU Hong-min,YANG Wan-yu,QIAO Bao-jian,ZHANG Ming,WANG Li-ya,LI Yan-long. Leek Pollen-tube Pathway Transgenic technique[J]. Shandong Agricultural Sciences, 2010, 0(3): 8-10 |
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| Authors: | ZHANG Wei LIU Hong-min YANG Wan-yu QIAO Bao-jian ZHANG Ming WANG Li-ya LI Yan-long |
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| Affiliation: | Key Laboratory of Biotechnology;Pingdingshan Academy of Agricultural Sciences;Pingdingshan 467001;China |
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| Abstract: | Leek transgene mediated by the pollen-tube pathway method is still at the research stage,which is a modern biotechnology combining the genetic engineering technique with the conventional hybridization breeding method.This paper described in detail the theoretical basis of foreign DNA fragments integrated into the leek genome and transformation of fertilization process,and the operating procedures,influence factors,advantages and disadvantages and prospects of the leek pollen-tube pathway transgenic techniqu... |
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| Keywords: | Pollen-tube pathway method Leek Transgenic breeding |
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