| 高粱总RNA的提取 |
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| 引用本文： | 段西飞,王静华,崔江慧,薛薇,李霄,常金华. 高粱总RNA的提取[J]. 华北农学报, 2008, 23(Z2) |
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| 作者姓名： | 段西飞 王静华 崔江慧 薛薇 李霄 常金华 |
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| 作者单位： | 河北农业大学,农学院,河北,保定,071000 |
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| 基金项目： | 科技厅博士资金项目(06547004D-2) |
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| 摘 要： | 介绍一种高粱总RNA的提取方法。该方法以CTAB为主要提取试剂,成功地提取出了高粱叶片的总RNA。获得的RNA条带清晰,A260/A280在1.8~2.0。这些结果表明提取的总RNA质量好,完整性好,可用于cDNA文库的构建、差异表达等工作。
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| 关 键 词： | 高粱 总RNA CTAB 提取 |
Total RNA Extraction from Sorghum |
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| DUAN Xi-fei,WANG Jing-hua,CUI Jiang-hui,XUE Wei,LI Xiao,CHANG Jin-hua. Total RNA Extraction from Sorghum[J]. Acta Agriculturae Boreali-Sinica, 2008, 23(Z2) |
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| Authors: | DUAN Xi-fei WANG Jing-hua CUI Jiang-hui XUE Wei LI Xiao CHANG Jin-hua |
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| Abstract: | A total RNA extraction method is introduced.The main extraction reagent is CTAB,the total RNA from sorghum leaves was successfully extracted.The RNA produced clear bands,and the ratios of A260/A280 were between 1.8 to 2.0.These results showed that the total RNA was of very good quality,very good integrity,and it can be used for the construction of cDNA library,diffrential display and so on. |
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| Keywords: | CTAB |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
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