滇润楠的组织培养及快速繁殖研究

 用滇润楠成年树带芽嫩茎、嫩叶和根部萌蘖枝条上带芽嫩茎为材料进行组织培养，在Ms+BA 4 mg·L^-1‘+NAA 0.5 mg·L^-1 的培养基上，树冠越冬芽褐化较轻，腋芽萌发率高，小苗生长良好，同时也有利于后期增殖；在MS+BA 8mg·L^-1+NAA 0.5 mg·L^-1 +KT 1.0 mg·L^-1的培养基上能分化出丛生芽，但增殖率低；在1/2 MS +NAA 0.1 mg·L^-1的培养基上诱导出白色健壮根。外植体取材部位、取材时间、激素等因素是引起滇润楠组培褐变及影响快速繁殖的主要原因。

Rapid Propagation of Machilus yunnanensis var.duclouxii in Vitro

The culture of the stem-bud from crown and stump shoot of Machilus yunnanensis var． duclouxii in vitro was studied．The results showed that the less browning and high germination rate of axially bud from the stem-bud appeared on the medium of MS+BA 4mg·L^-1+NAA 0.5 mg·L^-1，and the plan tlets grew well，which was beneficial to later mutipulcation．Th e tufted buds were diferentiated on the mediunl of MS+BA 8mg·L^-1 +NAA 0.5 mg·L^-1+KT 1.0 mg·L^-1，but multiplication rate was low．On the medium of 1/2 MS + NAA 0.1 mg·L^-1，the white healthy roots were induced ．The taken parts，taken-time of explants of Machilus yunnanensis var． duclouxii and hormone in medium are main factors that cause browning an d afect the speed of propagation during breeding．