RFLP/AFLP mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph1 in rice |
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| Authors: | P.N. Sharma Y. Ketipearachchi K. Murata A. Torii S. Takumi N. Mori C. Nakamura |
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| Affiliation: | (1) Laboratory of Plant Genetics, Department of Biological and Environmental Science, Faculty of Agriculture, and Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan;(2) Regional Agricultural Research and Development Center, Aralaqanwila, Sri Lanka;(3) Toyama Prefectural Agriculture Research Center, Yoshioka 1124-1, Toyama, Japan, 939-8153 |
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| Abstract: | We have constructed a linkage map of the rice brown planthopper (BPH)resistance gene, Bph1. RFLP and AFLP markers were selected by thebulked segregant analysis and used in the mapping study of 262 F2sthat were derived from a cross of `Tsukushibare', a susceptible japonica cultivar, and `Norin-PL3', an authentic japonicaBph1-introgression line. Twenty markers were mapped within a 28.9-cMregion containing the Bph1 locus on the long arm of rice chromosome12. Combining the result of segregation analysis of BPH resistance by themass seedling test and that of the markers, the Bph1 locus wasmapped within a 5.8-cM region between two flanking markers. The closestAFLP markers, em5814N and em2802N, was at 2.7 cM proximal to theBph1 locus. Together with the previously constructed high-resolutionmap of bph2 locating the locus at ca. 10 cM proximal to the Bph1 locus, this improved version of the linkage map would facilitatepyramiding these two important BPH resistance genes. |
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| Keywords: | brown planthopper (BPH) Bph1 and bph2 linkage map RFLP/AFLP markers rice (Oryza sativa L.) |
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