“芪蓝饮”及不同提取成分对NDV在鸡胚成纤维细胞中增殖的影响

为评价新兽药芪蓝饮及其不同提取成分的体外抗病毒活性,本试验通过细胞病变抑制法和MTT比色法检测了各提取成分对新城疫病毒(NDV)感染鸡胚成纤维细胞(CEF)过程的影响。结果显示,在3种加药方式中芪蓝饮原药(QLY)直接杀灭NDV的作用最强,其抑制率最高为128.85%;原药总多糖(TPS)干扰NDV的增殖作用最强,其抑制率最高为130.23%;原药总萜类(TT)直接杀灭NDV的作用最强,其抑制率最高为146.15%;板蓝根总生物碱(RIA)及其水溶性部位(RIAw)和酸性部位(RIAa)对抗NDV的方式是多环节的。综上所述,芪蓝饮及其提取成分表现出较强的干扰NDV增殖和直接杀灭NDV的作用,而阻断作用稍弱。

Effects of Qilanyin and its components on NDV proliferation in CEF

In order to observe the antivirus ability of the new animal drug ＂Qilanyin＂（QLY） and its components in vitro,cytopathic effect inhibition and MTT colorimetric assays were used to detect the antiviral ability of QLY and its components on Newcastle disease virus（NDV） in chick embryo fibroblast（CEF） cells.Results showed that QLY had the strongest ability of killing NDV directly,with the highest inhibition ratio of 128.85%.TPS was good at interrupting proliferation of NDV,the highest inhibition ratio was 130.23%.TT＇s killing NDV ability was the best,the highest inhibition ratio was 146.15%.However,RIA,RIAw and RIAa showed aitivirus ability in all the three means.In summary,QLY and its components showed better ability in interrupting proliferation of NDV and killing NDV,while the drugs was weak in blocking.