降钙素基因相关肽在鸭腔上囊胚胎及胚后发育过程中的表达

应用免疫组织化学技术研究降钙素基因相关肽在鸭腔上囊胚胎及胚后发育过程中的表达特征.结果显示,降钙素基因相关肽强阳性细胞出现于20 d胚龄至胚后14周龄的滤泡间上皮,20 d胚龄至胚后17周龄的小结相关上皮,新生雏至胚后14周龄的滤泡皮质,24 d胚龄至胚后14周龄的滤泡髓质,20 d胚龄至胚后14周龄的肌层和小动脉平滑肌.结果说明,降钙素基因相关肽表达于鸭腔上囊的不同部位及不同发育阶段;在腔上囊退化期,降钙素基因相关肽的表达表现出明显的变化特征,这可能是导致腔上囊退化的原因之一;滤泡间上皮与小结相关上皮在神经肽表达方面存在差异.

The expression pattern of calcitonin gene-related peptide in the duck bursa of fabricius during the embryonic and post embryonic development

The expression pattern of calcitonin gene-related peptide(CGRP)in the duck bursa of fabricius during the embryonic and post embryonic development was studied by the immunohistochemical method.The results showed that CGRP-positive cells with strong reaction were detected in the interfollicle epithelium(IFE).(from 20 days of age pre-hatching to 14 weeks of age post-hatching),the follicle-associated epithelium(FAE)(from 20 days prehatching to 17 weeks post-hatching),the follicle cortex(from hatching to 14 weeks post-hatching)and medullar(from 24 days pre-hatching to 14 weeks post hatching),the muscle cells in the muscular layer and small artery(from 20 days pre-hatching to 14 weeks post-hatching),respectively.The present results demonstrate that CGRP is expressed throughout the different compartments and at various stages of development in the duck bursa.CGRP expression presents obvious changes during the involution of the bursa,which may be one of the factors which involves in the regression of the organ.Moreover,IFE and FAE are different in their neuroendocrine function.