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| 致病性犬瘟热病毒CDV-JT1株的分离与鉴定 | |
| 引用本文： | 王秀东,谭斌,陈微晶,陈立志,武华.致病性犬瘟热病毒CDV-JT1株的分离与鉴定[J].中国兽医学报,2010,30(11). |
| 作者姓名： | 王秀东 谭斌 陈微晶 陈立志 武华 |
| 作者单位： | 1. 中国农业科学院,特产研究所,吉林,吉林,132109 2. 北京必威安泰生物科技有限公司,北京,100176 3. 中国农业科学院,特产研究所,吉林,吉林,132109;北京必威安泰生物科技有限公司,北京,100176 |
| 基金项目： | 国家科技支撑计划资助项目 |
| 摘 要： | 应用MDCK细胞从吉林某地犬瘟热(Canine distemper,CD)疑似发病犬肺脏组织中分离出1株病毒,该分离毒株经MDCK细胞传至第6代时出现典型的合胞体细胞病变(CPE)。经病毒形态观察、理化特性鉴定、RT-PCR和直接免疫荧光鉴定可知该分离毒株为犬瘟热病毒(CDV),命名为CDV-JT1;动物试验表明,试验犬接种CDV-JT1后21 d内都出现典型的犬瘟热症状;H基因序列分析表明,CDV-JT1株的H基因与中国分离株CDTaiChung株、TN株、SHLJ(07)1株、日本Hamamatsu株、Ueno株、Yanaka株和KDK-1株在基因型上同属于Asia-1型。CDV-JT1株含有包括CDV野毒株特有的309~311位在内的8个潜在的N-连接天冬酰氨糖基化位点。CDV-JT1强毒株的分离成功,对进一步开展疫苗研发、流行病学调查以及致病机理等方面的研究具有重要意义。 |
| 关 键 词： | 犬瘟热痛毒 强毒株 分离和鉴定 H基因 |
Isolation and identification of pathogenic canine distemper virus CDV-JT1 strain |
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| WANG Xiu-dong,TAN Bin,CHEN Wei-jing,CHEN Li-zhi,WU Hua.Isolation and identification of pathogenic canine distemper virus CDV-JT1 strain[J].Chinese Journal of Veterinary Science,2010,30(11). | |
| Authors: | WANG Xiu-dong TAN Bin CHEN Wei-jing CHEN Li-zhi WU Hua |
| Institution: | WANG Xiu-dong1,TAN Bin1,CHEN Wei-jing2,CHEN Li-zhi1,WU Hua1,2(1.Division of Zoonosis,Institute of Special Economic Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Jilin,Jilin 132109,China,2.Beijing Bigvet Biotech Co.,Ltd,Beijing 100176,China) |
| Abstract: | A virus strain was isolated by MDCK cells from lung of a dog with suspected CDV infection.The isolate could induce cytopathogenic effects of syncytia in MDCK cells after six passages.It was demonstrated to be CDV by morphology,physical and chemical properties,RT-PCR and direct immunofluorescent assay(FA) and was named CDV-JT1.Dogs artificially infected by CDV-JT1 developed symptom of CD during 21 days after infection.The H gene of the virus was sequenced and showed that the isolate,together with CDTaiChung,... |
| Keywords: | canine distemper virus virulent isolation and identification H gene |
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