| 大熊猫源肺炎双球菌的分离鉴定 |
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| 引用本文： | 王春花,李德生,王承东,刘孟良,汤纯香,吴虹林,朱玲.大熊猫源肺炎双球菌的分离鉴定[J].黑龙江畜牧兽医,2012(1):85-87. |
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| 作者姓名： | 王春花 李德生 王承东 刘孟良 汤纯香 吴虹林 朱玲 |
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| 作者单位： | 四川农业大学动物医学院;四川农业大学动物疫病与人类健康实验室;中国大熊猫保护中心 |
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| 基金项目： | 长江学者和创新团队发展计划创新团队项目(IRT0848) |
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| 摘 要： | 为了分析、丰富表现呼吸道症状的患病大熊猫的病原病因学,选用有效药物预防控制该类疾病,试验对从中国大熊猫保护中心采集的患病大熊猫鼻腔拭子进行了细菌培养、菌落形态学观察、生化试验、溶血试验和动物致病性试验。结果表明:分离到致病性肺炎双球菌;分离菌对万古霉素、青霉素、氨苄西林、红霉素、羧苄青霉素和阿莫西林等高度敏感,对丙氟哌酸、卡那霉素、四环素等中度敏感,对链霉素、强力霉素等药物有耐药性。
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| 关 键 词： | 大熊猫 肺炎双球菌 分离 鉴定 药敏试验 |
Isolation and identification of Streptococcus pneumoniae from diseased giant pandas |
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| WANG Chun-hua,LI De-sheng,WANG Cheng-dong,LIU Meng-liang,TANG Chun-xiang,WU Hong-lin,ZHU Ling.Isolation and identification of Streptococcus pneumoniae from diseased giant pandas[J].Heilongjiang Animal Science And veterinary Medicine,2012(1):85-87. |
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| Authors: | WANG Chun-hua LI De-sheng WANG Cheng-dong LIU Meng-liang TANG Chun-xiang WU Hong-lin ZHU Ling |
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| Institution: | 1,2(1.College of Veterinary Medicine,Sichuan Agricultural University,Ya’an 625014,China; 2.Laboratory of Animal Disease and Human Health,Sichuan Agricultural University, Ya’an 625014,China;3.China Conservation and Research Center for the Giant Panda,Wolong 623006,China) |
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| Abstract: | The cotton swab with pathogenic bacteria were obtained through the nasal cavity of several diseased giant pandas fed in China Conservation and Research Center for the Giant Panda,then used for the bacteria cultivation,observing colony morphology,biochemical test,the hemolytic test and the animal pathogenicity test.The results showed that the pathogenic bacteria was isolated and identified as Streptococcus pneumoniae,the isolated Streptococcus pneumoniae was highly sensitive to vancomycin,chloromycetin,penicillin,ampicillin,erythromycin,carbencillin,amoxicillin and etc.It was moderate sensitive to ciprofloxacin,kanamycin,tetracycline,showing a resistance against streptomycin and doxycycline. |
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| Keywords: | giant pandas Streptococcus pneumoniae isolation identification drug susceptibility test |
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