| 摘 要： | <正>The experiment was conducted to study the effects and possible mechanism of GLP-2 on proliferation,metabolism and apoptosis of cultured enterocytes from a 28-d weaned piglet injured by exposure to β-conglycinin.A cell damage model was established to investigate cell proliferation, metabolism and apoptosis by exposing primary cell cultures of intestinal epithelial cells(IEC) to 1.2 and 2.4 mg/mL β-conglycinin.A 2×3 factorial experiment was then used to study the effect of different GLP-2 concentrations of(1×10~(-9),1×10~(-8) and 1×10~(-7)mol/L),in combination with the two concentrations ofβ-conglycinin.Cells exposed to the allergenβ-conglycinin had decreased(P0.05) MTT OD;decreased (P0.01) protein retention and total protein content of cells;increased(P0.01) LDH and caspase-3 activities and decreased(P0.05) Na~+,K~+-ATPase activity.When GLP-2 was used in combination withβ-conglycinin,MTT OD,protein retention,total protein content and Na~+,K~+-ATPase activity significantly increased(P0.05);LDH activity gradually decreased(P0.05 or P0.01) and Caspase-3 activity significantly decreased(P0.01) with increasing concentrations of GLP-2.The results indicated thatβ-conglycinin had adverse effects on proliferation and integrity of IEC in vitro.GLP-2 relieved or prevented the adverse effects ofβ-conglycinin on proliferation and integrity of IEC by regulating Na~+,K~+- ATPase and Caspase-3 activities,and consequently affecting cell metabolism.
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