吉他霉素、泰乐菌素和金霉素对耐药性大肠杆菌生长代谢和耐药性累积的影响

利用体外诱导猪源大肠杆菌产生耐药性的方法,比较研究了防病促生长剂量的吉他霉素(20mg/L)、金霉素(100mg/L)、泰乐菌素(20mg/L)对耐药型和野生型致病大肠杆菌耐药性累积、蛋白质氨基酸代谢、能量利用和代谢调节的影响。结果表明:产生耐药性后的大肠杆菌在防病促生长剂量环境中继续培养,不影响最小抑菌值(MIC)和最小杀菌浓度(MBC);比野生型大肠杆菌显著或极显著提高菌液总蛋白和总AA产量(P<0.05)、乳酸产量(P<0.05)、Na+-K+-ATP酶活性(P<0.05)和NO产量(P<0.01)。由此可得出,猪源大肠杆菌产生耐药性以后,继续使用饲用抗生素不会进一步增加耐药性,但显著增强能量、蛋白代谢。

Influence of Kitasamycin,Tylosin and Chiortetracycline on E.coli Resistance Strain Metabolism and Drug Resistance Accumulation

Induce cultivating in E. coli resistance strain to research the influence of disease prevention dose Kitasamycin (20 mg/L), tylosin (20 mg/L) and Chlortetracycline (100 mg/L) on E. coli drug resistance, protein Metabolism, energy utilization and metabolism regulation in E. coli resistance and wild strain. Results show that the drug resistance was not increased when E. coli resistance strain was cultivated in disease prevention dose antibiotics, MIC and MBC did not change, While total protein and total AA yield significantly (P < 0.05),LD yield (P <0.05),Na+-K+-ATPapes activity and NO yield (P < 0.01)were increased.In conclusion, it can't increase the drug resistance when antibiotics was used sequencely in E. coli resistance strain, but increase protein and energy metabolis significantly.