贵州地方猪脂肪特异蛋白27基因SNPs筛查与生物信息学分析

以3个贵州地方猪种(可乐猪、贵州白香猪、黔北黑猪)为试验素材构建品种DNA池,采用直接测序技术对猪脂肪特异蛋白27(fat-specific protein 27,Fsp27)基因的第4~5外显子区域进行SNPs快速筛查,共检测出4个SNPs位点:intron3-T2169C,exon4-G5A,intron4-G21C和exon5-C5G,其中exon4-G5A和exon5-C5G使编码氨基酸发生Arg→Gln,Thr→Ser的改变。进一步的生物信息学分析显示,exon4-G5A位点的变异导致mRNA的二级结构改变,exon5-C5G多态位点使蛋白质的二级结构发生变化,且当exon4-G5A和exon5-C5G位点的碱基分别为A和G时蛋白质三级结构与其它3种碱基组合(G与G,G与C,A与C)的三级结构明显不同。

SNPs Screening and Bioinformatics Analysis of Fsp27 Gene in the Breeds of Guizhou Local Pigs

Three breeds of pig(Kele pig,Guizhou White Xiang pig and Qianbei Black pig) were used to constructed DNA pooling,the SNP in exon4 to exon5 of Fsp27 gene were rapidly screened by DNA sequencing.Four SNPs were detected in the three breeds of pig totally,they were intron3-T2169C,exon4-G5A,intron4-G21C and exon5-C5G,the exon4-G5A and exon5-C5G sites led to coding amino acids change from Arginine to Glutarnine,from Threoine to Serine respectively.The results of the bioinformatics analysis indicated that the mutation in loci exon4-G5A could cause the change of mRNA secondary structure,while the exon5-C5G site led to the protein secondary structure change,when the locus was A,G in the exon4-G5A and exon5-C5G respectively the protein tertiary structure would be different from other(G and G,G and C,A and C) base combinations.