锦州地区副猪嗜血杆菌分离鉴定与药敏试验

从锦州市某猪场7份疑似副猪嗜血杆菌病料中分离到4株革兰氏阴性细小杆菌,进行了细菌培养特性试验、纯化后镜检、V因子需要试验、卫星现象检查、生化试验、PCR鉴定、药物敏感试验、细菌的致病性试验。结果表明,分离的菌株为副猪嗜血杆菌,4株分离菌株对林可霉素、环丙沙星、强力霉素、头孢噻肟、氧氟沙星和恩诺沙星均较敏感;对卡那霉素、复方新诺明、克林霉素、金霉素、四环素均为耐药;对链霉素呈现不同程度的敏感性。

Isolation and Identification of Drug Sensitivity Test of Isolated Haemophilus parasuis in Jinzhou

4 strains of gram negative bacilli were isolated from 7 portion materils colleted from some pig farms of Jinzhou. Haemophilus parasuis was finally diagnosised by isolated culture test, staining microscopy, biochemical tests, the test of V factor need, satellite phenomenon examination and PCR.The results of drug sensitivity test indicated that 4 strains of bacteria were sensitive to lincomycin, ciprofloxacin, doxycycline, cefotaxime,ofloxacin,and enrofloxacin and resistant to kanamycin, cotrinoxazole, clindamycin, chlortetracycline and tetracycline. 4 strains of bacteria showed different sensitivity to streptomycin.