猪源芽孢杆菌的分离鉴定及生物学特性研究

从自然生长的健康猪肠道中分离到13株芽孢杆菌,通过生理生化试验初步鉴定其中9株为枯草芽孢杆菌,2株为巨大芽孢杆菌,1株为地衣芽孢杆菌,1株为凝结芽孢杆菌;对这13株芽孢杆菌分别进行了耐酸性试验、耐胆盐试验、体外抑菌试验和动物安全性试验。结果表明,其中1株地衣芽孢杆菌B7株和1株枯草芽孢杆菌B9株均有较强的耐酸、耐胆盐能力,对致病性大肠杆菌具有较好的体外抑制能力,且均安全无毒,可作为动物用益生菌制剂的候选菌种。

Isolation,Identification of Bacillaceae from Swine and Study on its Biological Characteristics

We had isolated thirteen bacterial strains belonging to Bacillaceae from natural growth of healthy pigs intestin,and preliminary identified that nine strains of those was Bacillus subtilis,two strains of those was Bacillus megaterium,one strain of those was Bacillus licheniformis and one strain of those was Bacillus coagulans through the physiological and biochemical tests.And then we conducted experiments of acid resisting test,bile salt resisting test,antibacterial test in vitro and animal safety trials on the thirteen bacterial strains.The results showed that one strain of the Bacillus licheniformis named B7 and one strain of the Bacillus subtilis named B9 had intense acid,bile salt-resistant ability,and strong antibacterial effect in vitro of pathogenic Escherichia coli,and they were nonpoisonous.So,B7 and B9 could be as candidate species of veterinary probiotic.