2株猪繁殖与呼吸综合征病毒全基因序列分析

利用RT-PCR分段扩增的方法,扩增出猪繁殖与呼吸综合征病毒(PRRSV)中山分离株(ZS-GD株)、珠海分离株(ZH-GD株)覆盖全长基因组的10个片段,分别克隆入pMD18-T载体进行序列测定,获得了PRRSV ZS-GD株和ZH-GD株基因组全长15320 bp的核苷酸序列。利用生物信息学软件对PRRSV ZS-GD株和ZH-GD株全基因组序列进行了遗传进化分析,结果显示PRRSV ZS-GD株和ZH-GD株与美洲型参考株的核苷酸序列相似性分别为88.1%~97.8%和88.4%~98.0%;与欧洲型参考株(Lelystad)的核苷酸序列相似性分别为60.6%和60.4%,且在2株毒的Nsp2蛋白上有30个aa的缺失,表明PRRSV ZS-GD株和ZH-GD株均为美洲型分离株的缺失变异株。

Sequence Analysis of Complete Genome of Two Strains of Porcine Reproductive and Respiratory Syndrome Virus

Ten gene fragments contains two strains of porcine reproductive and respiratory syndrome virus which were designated ZS-GD strain and ZH-GD strain complete genome were amplified respectively by RT-PCR, and cloned into pMD18-T vector for sequencing. Phylogenetic analysis of two strains of PRRSV complete genome sequence by bioinformatics software showed that the complete genome of ZS-GD and ZH-GD were 15320 bp, the genome of ZS-GD strain and ZH-GD strain shared 88.1% to 97.8% and 88.4% to 98.0% of nucleotide homology with American type strain, respectively; shared 60.6% and 60.4% of nucleotide homology with Europe type strain(Lelystad virus). There were 30aa depletion in Nsp2 protein of two stains. The results showed that these two strains were depletion and variant strain of American type.