| 人工设计天蚕素抗菌肽AMP-D及其活性测定 |
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| 引用本文： | 韩晋辉,翟培,施用晖.人工设计天蚕素抗菌肽AMP-D及其活性测定[J].中国畜牧兽医,2012,39(7):159-162. |
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| 作者姓名： | 韩晋辉 翟培 施用晖 |
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| 作者单位： | 1. 广东食品药品职业学院,广东广州,510520
2. 江南大学,江苏无锡,214122 |
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| 基金项目： | 广东食品药品职业学院院级资助项目 |
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| 摘 要： | 本试验通过对抗菌肽结构改造优化,得到了一条新设计的抗菌肽AMP-D,并利用固相合成技术人工合成后对其活性和溶血性进行测定。试验结果表明,AMP-D对革兰氏阴性菌及真菌没有生物活性,对革兰氏阳性菌有明显的抑制作用,且其没有溶血活性;AMP-D对金黄色葡萄球菌的最小抑菌浓度为10.5 μg/mL,较原设计肽活性有较大提高。
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| 关 键 词： | 天蚕素 AMP-D 抑菌活性 溶血性 |
| 收稿时间： | 2012-05-18 |
Artificial Design Cecropins of AMP-D and Determination of Activity |
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| HAN Jin-hui
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ZHAI Pei
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SHI Yong-hui.Artificial Design Cecropins of AMP-D and Determination of Activity[J].China Animal Husbandry & Veterinary Medicine,2012,39(7):159-162. |
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| Authors: | HAN Jin-hui ZHAI Pei SHI Yong-hui |
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| Institution: | 1. Guangdong Food and Drug Vocational College, Guangzhou 510520,China;2. Jiangnan University,Wuxi 214122,China |
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| Abstract: | By the experiment of antimicrobial peptides structure optimization and solid-phase synthesis technology of synthetic,new antibacterial peptide AMP-D could be obtained. According to determination of its activity and hemolytic, the experimental results showed that AMP-D could inhibit the growth of Gram-positive bacteria, and was no hemolytic activity. Minimal inhibitory concentration of AMP-D was 10.5 μg/mL against Staphylococcus aurous. The antimicrobial activities of AMP-D had more improve than the original peptide. |
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| Keywords: | cecropin AMP-D antibacterial activity hemolytic |
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