绵羊卵丘细胞对裸卵体外成熟及孤雌发育影响的研究

本研究探讨绵羊卵丘细胞对裸卵体外成熟和后期发育的影响。实验分为4组,对照组为卵丘-卵母细胞复合体(COCs);裸卵(DO)组;COCs与DO共培养组(CODO);卵丘细胞(CC)与DO共培养组(CCDO)。体外成熟培养18 h后,检测各组卵子成熟质量并进行孤雌发育研究。结果表明:CODO和CCDO组中裸卵活率显著高于DO组(P<0.05),但显著低于COCs组(P<0.05),且COCs组中卵子活率极显著高于DO组(P<0.01);在极体排出率方面,CODO、CCDO和DO3组差异不显著(P>0.05),但是它们都显著低于COCs组(P<0.05)。COCs、CODO、CCDO和DO 4组卵裂率差异均不显著(P>0.05)。然而,DO组的囊胚率显著低于CODO和CCDO组(P<0.05),极显著低于COCs组(P<0.01),但CODO和CCDO 2组中裸卵的囊胚率差异不显著(P>0.05)。总之,无论散在的卵丘细胞还是COCs均能提高裸卵体外成熟及后期发育潜力。

Effects of Sheep Cumulus Cell on the Maturation and Developlment of Denuded Oocytes in vitro

The present study discussed the effects of sheep cumulus cells on maturation and development of denuded oocyts in vitro.The experiment was divided into four groups: Control,cumulus-oocytes complexes(COCs);denuded oocytes(DO);COCs and DO co-culture group(CODO) and cumulus cell(CC)and DO co-culture group(CCDO).After being cultured for 18 h in vitro,mature quality of oocytes in four groups was checked,and also the lived oocytes were parthenogenetically activated for the further study.Our results showed that the live rates in CODO and CCDO groups were significant higher than that in DO group(P<0.05),However,the rates in CODO,CCDO and DO were all significantly lower than that in COCs group(P<0.05).For the extruding rates of the first polar body of oocytes,CODO,CCDO and DO had no significant differences(P>0.05),but they were all significantly lower than that of control(P<0.05).Nevertheless,blastula rates in DO was significantly lower than that in COCs(P<0.05).Although there was no significant differences in blastula rates between CODO and CCDO groups(P>0.05),the rates in the two groups were both significantly higher than that in DO group(P<0.05).Anyways,cumulus cell and COCs both could improve maturation quality and developmental potentialities of denuded oocytes during maturing culture in vitro.