| 猪黑素皮质素受体-4基因在成纤维细胞中的沉默研究 |
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| 引用本文： | 张冬杰,汪亮,刘娣.猪黑素皮质素受体-4基因在成纤维细胞中的沉默研究[J].中国畜牧兽医,2012,39(7):147-150. |
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| 作者姓名： | 张冬杰 汪亮 刘娣 |
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| 作者单位： | 黑龙江省农业科学院畜牧研究所,黑龙江哈尔滨 150086 |
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| 基金项目： | 转基因生物新品种培育重大专项 |
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| 摘 要： | 黑素皮质素受体-4(melanocortin receptor-4,MC4R)、黑素皮质素受体-3(melanocortin receptor-3,MC3R)、瘦素(Leptin)和缩胆囊素受体(cholecystokinin A receptor,CCKAR)基因均是与采食量相关的功能基因,为了研究这4个基因之间可能存在的互作关系,本研究采用RNAi方法,将体外培养的成纤维细胞中的MC4R基因沉默,然后采用Real-time PCR方法对Leptin、MC3R和CCKAR基因的表达变化情况进行了研究。结果表明,MC4R被沉默后,Leptin、MC3R和CCKAR的表达水平均没有发生显著变化。
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| 关 键 词： | 猪 采食量 MC4R RNAi Real-time PCR |
Study on Silencing of MC4R Gene in Pig Fibroblasts |
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| ZHANG Dong-jie
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WANG Liang
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LIU Di.Study on Silencing of MC4R Gene in Pig Fibroblasts[J].China Animal Husbandry & Veterinary Medicine,2012,39(7):147-150. |
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| Authors: | ZHANG Dong-jie WANG Liang LIU Di |
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| Institution: | Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China |
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| Abstract: | MC4R,MC3R,Leptin and CCKAR are all associated with feed intake.To analysis the potential relationship of these four genes,MC4R gene was silenced in fibroblasts by RNAi method,then Real-time PCR method was used to detect the expression changes of Leptin,MC3R and CCKAR.The results showed that,after MC4R was silenced,Leptin,MC3R and MC4R expression level were not changed. |
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| Keywords: | pig feed intake MC4R RNAi Real-time PCR |
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