不同来源寡糖和小肽对饲喂高脂日粮小鼠小肠绒毛形态的影响

试验主要通过建立高脂动物模型诱导小鼠消化道氧化应激,研究不同来源的小肽和寡糖对小鼠体内小肠绒毛形态结构的影响。选取72只雄性ICR小鼠,体重为(24±2)g,随机分为6组(n=12),分别饲喂基础日粮组(对照组)、高脂日粮组、0.5%胶原蛋白肽(CP)高脂日粮组、0.5%米蛋白肽(RP)高脂日粮组、0.5%甘露寡糖(MOS)高脂日粮组和0.5%壳寡糖(COS)高脂日粮组。6周后处死小鼠,用HE染色制作石蜡切片,光镜观察各组小鼠小肠组织结构的变化。结果表明,饲喂高脂日粮使小鼠小肠绒毛脱落严重,绒腺比变小。添加0.5% CP、0.5% RP、0.5% MOS和0.5% COS的高脂日粮组均能减轻小肠绒毛短缩融合、脱落现象,使高脂小鼠小肠绒毛有所增长,隐窝深度变浅,绒腺比增大,其中0.5% CP和0.5% MOS对肠道黏膜保护效果最好。

Effect of Different Oligosaccharides and Peptides on the Small Intestinal Villi Histology of Mice Fed with High-fat Diet

This study was conducted to investigate the effect of different oligosaccharides and small peptides on the small intestinal villi histology of mice fed with high-fat diet.Seventy-two male ICR mice with initial average body weight of 24 g±2 g were randomly divided into 6 groups,which were fed with basal diet,high-fat diet,high-fat diet with 0.5% CP,0.5% RP,0.5% MOS and 0.5% COS,respectively.After 6 weeks,mice were killed to observe the mice’s small intestines in each group which were HE stained by light microscopy.The mice fed with high-fat diet lost small intestinal villus seriously and had a lower ratio of villus height and crypt depth than the control.All the high-fat diets with 0.5% CP,0.5% RP,0.5% MOS and 0.5% COS could ease the phenomenon of small intestinal villus’s shedding and short fusion,which not only increased the height of small intestinal villus,the ratio of villus height and crypt depth,but also shallowed small intestinal crypt depth compared with the high-fat diet groups.The protective effect of high-fat diet groups with 0.5% CP and 0.5% MOS on the protection of intestinal mucosa were the best.