| 不同消化酶对羊驼皮肤黑素细胞分离的影响 |
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| 引用本文： | 白瑞,于志慧,杨刚,王海东,范瑞文,董常生.不同消化酶对羊驼皮肤黑素细胞分离的影响[J].中国畜牧兽医,2012,39(8):136-138. |
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| 作者姓名： | 白瑞 于志慧 杨刚 王海东 范瑞文 董常生 |
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| 作者单位： | 山西农业大学动物科技学院,山西太谷 030801 |
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| 基金项目： | 国家自然基金，山西农业大学科技创新基金 |
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| 摘 要： | 本研究旨在探讨羊驼皮肤黑素细胞的分离、培养及鉴定方法,为进一步研究色素沉积提供合适的细胞模型。取1~3岁的青年羊驼皮肤,用胰酶、胰酶∶EDTA、DispaseⅡ酶3种不同的消化酶消化羊驼皮肤,再用胰酶∶EDTA消化,分离得到羊驼黑素细胞,接种于多种促进黑素细胞生长的营养成分DMEM/F12培养基中,通过观察黑素细胞的生长、细胞接种数和细胞贴壁数检验黑素细胞的纯度。结果表明,DispaseⅡ组处理的羊驼皮肤,分离得到的黑素细胞明显多于其它2个组(P＜0.01)。综上所述,选用DispaseⅡ酶作为分离羊驼皮肤的消化酶,可获得较纯的黑素细胞。
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| 关 键 词： | 羊驼 黑素细胞 DispaseⅡ酶 纯化 |
| 收稿时间： | 2011-12-27 |
Effects of Different Enzymes on the Melanocyte Isolation of Alpaca Skin |
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| BAI Rui
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YU Zhi-hui
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YANG Gang
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WANG Hai-dong
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FAN Rui-wen
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DONG Chang-sheng.Effects of Different Enzymes on the Melanocyte Isolation of Alpaca Skin[J].China Animal Husbandry & Veterinary Medicine,2012,39(8):136-138. |
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| Authors: | BAI Rui YU Zhi-hui YANG Gang WANG Hai-dong FAN Rui-wen DONG Chang-sheng |
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| Institution: | College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China |
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| Abstract: | In order to explore and establish a new method of isolation,culture,and identification of alpaca skin melanocyte in vitro for further studying to provide a suitable pigment deposition cell model.Take 1 to 3 years young alpacal skin,with three different enzymes(trypsin,trypsin-EDTA,Dispase Ⅱ) digestion alpaca skin,alpaca melanocyte were obtained by trypsin-EDTA digestion,placed into DMEM/F12 culture medium.Analyze melanocyte growth,cell yields and cells attached.The results showed Dispase Ⅱ group had a significant higher other groups(P<0.01).In summary,alpaca melanocyte were obtained by Dispase Ⅱ digestion. |
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| Keywords: | alpaca melanocyte DispaseⅡ depuration |
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