| 猪源戊型肝炎病毒ORF2部分基因的原核表达及其鉴定 |
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| 引用本文： | 欧阳昀,张亮权,刘志刚,齐海涛,徐廷川,张民泽,张超逸,张桂红.猪源戊型肝炎病毒ORF2部分基因的原核表达及其鉴定[J].中国畜牧兽医,2012,39(6):86-89. |
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| 作者姓名： | 欧阳昀 张亮权 刘志刚 齐海涛 徐廷川 张民泽 张超逸 张桂红 |
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| 作者单位： | 华南农业大学兽医学院,广东广州 510642 |
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| 基金项目： | 国家生猪现代农业产业体系 |
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| 摘 要： | 本试验旨在表达猪源戊型肝炎病毒(sHEV)ORF2部分基因片段,纯化表达产物并进行抗原性分析。以sHEV的cDNA为模板,PCR扩增ORF2基因片段,构建重组质粒pET32a-dORF2,转化表达菌BL21,IPTG诱导,SDS-PAGE凝胶电泳和Western blotting进行检测。试验结果表明,获得60 ku的目的蛋白,该蛋白具有良好的抗原性,主要以可溶性形式存在。
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| 关 键 词： | 戊型肝炎病毒 ORF2基因 重组质粒 原核表达 |
Swine Hepatitis E Virus ORF2 Partial Gene Expression and Identification of Prokaryotic |
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| OUYANG Yun
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ZHANG Liang-quan
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LIU Zhi-gang
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QI Hai-tao
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XU Ting-chuan
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ZHANG Min-ze
,
ZHANG Chao-yi
,
ZHANG Gui-hong.Swine Hepatitis E Virus ORF2 Partial Gene Expression and Identification of Prokaryotic[J].China Animal Husbandry & Veterinary Medicine,2012,39(6):86-89. |
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| Authors: | OUYANG Yun ZHANG Liang-quan LIU Zhi-gang QI Hai-tao XU Ting-chuan ZHANG Min-ze ZHANG Chao-yi ZHANG Gui-hong |
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| Institution: | College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642, China |
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| Abstract: | The assay was aim to express swine hepatitis E virus(sHEV) ORF2partial gene fragments,purify the expression product and analyze the antigenicity.ORF2gene was amplified by PCR from sHEV cDNA as a template,and the recombinant plasmid pET32a-dORF2was constructed,then transformed into the expression bacteria BL21.After IPTG induction,the protein was analyzed by SDS-PAGE and Western blotting.The results showed that the protein was expressed fromORF2gene in the form of soluble,the protein was 60ku.The result of Western blotting analysis indicated that the protein could react with the HEV positive serum. |
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| Keywords: | hepatitis E virus ORF2gene recombinant plasmid prokaryotic expression |
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