| 海藻糖生物特性及其在ELISA技术研发中的应用 |
| |
| 引用本文： | 王磊,崔东安,张景艳,李建喜.海藻糖生物特性及其在ELISA技术研发中的应用[J].中国畜牧兽医,2012,39(10):69-72. |
| |
| 作者姓名： | 王磊 崔东安 张景艳 李建喜 |
| |
| 作者单位： | 中国农业科学院兰州畜牧与兽药研究所,农业部新兽药工程重点实验室, 甘肃省中兽药工程技术研究中心,甘肃兰州 730050 |
| |
| 基金项目： | 甘肃省科技支撑计划项目，中央级公益性科研院所基本科研业务费专项资金项目 |
| |
| 摘 要： | 海藻糖属非还原性双糖,在生物体内作为一种贮藏性糖类,可提供能量来源,同时它也是一种重要的应激代谢产物,对应激状态具有高度抗性。外源性海藻糖对细胞、抗体等生物活性物质同样具有非特异性生物保护作用。文章综述了海藻糖的能量储备、抗应激、细胞稳定剂等生物学特性,以及对细胞、抗体和ELISA中包板抗原或抗体的保护作用,并对其在ELISA技术改善中的应用前景做了推测与展望。
|
| 关 键 词： | 海藻糖 生物学特性 ELISA |
| 收稿时间： | 2012-02-29 |
Biological Properties of Trehalose and its Application in ELISA |
| |
| WANG Lei
,
CUI Dong-an
,
ZHANG Jing-yan
,
LI Jian-xi.Biological Properties of Trehalose and its Application in ELISA[J].China Animal Husbandry & Veterinary Medicine,2012,39(10):69-72. |
| |
| Authors: | WANG Lei CUI Dong-an ZHANG Jing-yan LI Jian-xi |
| |
| Institution: | Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutics of CAAS, Lanzhou 730050,China |
| |
| Abstract: | Trehalose is a non-reducing disaccharide whose function in some organisms is as a source of energy or a protectant against the stress. The exogenous trehalose is contributed to protecting the bioactivitie of some substances like cells and antibodies in vitro. This review summarizes the biological properties of trehalose, such as energy source, stress resistance, cell stabilizer and helping to stabilize antigens or antibodies in ELISA. The trehalose is also helpful for improving ELISA detection. |
| |
| Keywords: | trehalose biological properties ELISA |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
