蝗虫多肽对大鼠肥胖抑制作用的研究

试验旨在研究能量型日粮中添加蝗虫多肽对大鼠肥胖及脂肪代谢的影响。试验选用30只6周龄的Wistar- Imamichi系雄性大鼠,随机分为3组,试验1组在基础日粮中添加20%猪油;试验2组在基础日粮中添加20%猪油+添加10%蝗虫多肽粉末;试验3组在基础日粮中添加20%猪油+添加10%纤维素粉,试验期8个月。试验期间每周末称重一次,试验结束后测定血液生化指标及各脏器中瘦素(Leptin)浓度,并运用Real-time PCR技术对脂肪中瘦素mRNA表达量进行了测定。结果表明,试验2组与试验1、3组相比,从18周龄开始体重增加明显减缓,对附睾周围脂肪重量存在极显著的抑制(P＜0.01),各脏器重量未因使用蝗虫多肽而变化;部分血液生化指标存在显著或极显著差异(P＜0.05,P＜0.01);试验2、3组的血清、脑脊髓液中瘦素浓度极显著低于试验1组(P＜0.01);试验2组瘦素mRNA表达量显著高于试验1组(P＜0.05)。说明蝗虫多肽所含多种功能性酶能够抑制大鼠体内脂肪的增加,有效地控制了肥胖。

Study on Inhibition of Huang Chong Polypeptide in Obese Wistar Rats

In order to explore the effect of dietary Huang Chong polypeptide (HCP) on fat and lipids metabolism in Wistar rats. 30 Wistar rats at 6 weeks were randomly allotted into 3 groups. We fed the basal diet which added 20% lard to the first group, and added 10% HCP, 10% fibre based on the first group diet to groups 2 and 3. The experiment period lasted for 8 months. Body weights were detected every weekend, and serum biochemical parameters and Leptin concentration were evaluated by the end of the experiment. The Real-time PCR method was used to dected the expression changes of Leptin mRNA in lipids. The results showed that the body weight of group 2 increased slower than groups 1 and 3 from 18 weeks, the fatty weight of epididymis were inhibited significantly in group 2(P＜0.01), viscera weights were no changed in groups 1, 2 and 3. The serum biochemical parameters of T-Bil, AST, ALT, ALP, BUN and Na⁺ had significant difference(P＜0.05,P＜0.01). The Leptin concentration of groups 2 and 3 were significantly lower than group 1 in serum and cerebrospinal fluid (CSF) (P＜0.01). The Leptin mRNA expression of group 2 was significantly higher than group 1(P＜0.05). The results indicated that Huang Chong polypeptide could inhibit and control fatty in rats.