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| 兔防御素基因NP-1的克隆及其在大肠杆菌中的表达 | |
| 引用本文： | 秦晓玉,彭章华,董晓,苗向阳,罗庆苗. 兔防御素基因NP-1的克隆及其在大肠杆菌中的表达[J]. 中国畜牧兽医, 2012, 39(9): 7-11 |
| 作者姓名： | 秦晓玉 彭章华 董晓 苗向阳 罗庆苗 |
| 作者单位： | 1. 中国农业科学院北京畜牧兽医研究所,北京 100193; 2. 青岛农业大学,山东青岛 266109; 3. 四川泸县畜牧局,四川泸州 646100 |
| 基金项目： | 国家高技术研究发展863计划项目，中国农业科学院创新基金 |
| 摘 要： | 选用大肠杆菌偏嗜性的密码子对兔防御素基因NP-1进行改造,人工合成编码NP-1成熟肽的基因片段,并将其克隆到T-easy载体上,再经双酶切后重组于原核表达载体pMAL-p2X。经IPTG诱导后,在大肠杆菌TB1中融合表达出兔防御素NP-1成熟肽。 |
| 关 键 词： | 畜牧学 防御素 融合蛋白 表达 |
| 收稿时间： | 2012-03-13 |
Artificial Synthesis of Rabbit Defensin NP-1 Gene and its Expression in E.coli |
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| QIN Xiao-yu , PENG Zhang-hua , DONG Xiao , MIAO Xiang-yang , LUO Qing-miao. Artificial Synthesis of Rabbit Defensin NP-1 Gene and its Expression in E.coli[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(9): 7-11 | |
| Authors: | QIN Xiao-yu PENG Zhang-hua DONG Xiao MIAO Xiang-yang LUO Qing-miao |
| Affiliation: | 1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. Qingdao Agricultural University, Qingdao 266109, China; 3. Sichuan Luxian Country Animal Husbandry Bureau, Luzhou 646100,China |
| Abstract: | The mature peptide sequence of rabbit defensin NP-1 gene was modified with favour codes for E.coli and synthesized chemically.The target gene fragment modified was cloned into vector T-easy,and then inserted into fusion expression plasmid pMAL-p2X through digesting of two restriction enzymes.The positive recombinant expression vector pMAL-NP-1 was transformed into E.coli TB1,which could effectively produce the fusion protein MBP and NP-1 induced with IPTG. |
| Keywords: | animal science defensin fusion protein expression |
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