鸡新城疫、传染性支气管炎、禽流感(H9亚型)三联灭活 疫苗(La Sota株+M41株+SS/94株)免疫程序研究

设计不同的免疫程序,用鸡新城疫、传染性支气管炎、禽流感(H9亚型)三联灭活疫苗(La Sota株+M41株+SS/94株)免疫黄羽肉鸡,通过对ND、IB、H9抗体滴度监测,探讨ND、IB、H9抗体消长规律及鸡新城疫、传染性支气管炎、禽流感(H9亚型)三联灭活疫苗(La Sota株+M41株+SS/94株)的免疫程序。试验结果表明,用鸡新城疫、传染性支气管炎、禽流感(H9亚型)三联灭活疫苗(La Sota株+M41株+SS/94株)免疫黄羽肉鸡后,其诱导产生的ND、IB、H9抗体的消长规律基本同步;仅于10日龄免疫一次三联灭活苗,其抗体水平较低,于20、40日龄二免、三免或10日龄先用鸡新城疫病毒(La Sota株)、禽流感病毒(H9亚型,SS/94株)二联灭活疫苗作基础免疫,20或40日龄再用三联灭活苗作加强免疫,则上述3种抗体均快速上升,且维持时间长。根据试验结果,建议按照正常免疫程序作基础免疫的健康肉鸡,饲养期较短的可于20日龄左右用鸡新城疫、传染性支气管炎、禽流感(H9亚型)三联灭活疫苗(La Sota株+M41株+SS/94株)作加强免疫,0.3 mL/只;饲养期较长的则于40日龄左右用鸡新城疫、传染性支气管炎、禽流感(H9亚型)三联灭活疫苗(La Sota株+M41株+SS/94株)作加强免疫,0.5 mL/只。

Immunization Program of Newcastle Disease, Infectious Bronchitis and Avian Influenza(H9 Subtype)Vaccine, Inactivated(Strain La Sota +Strain M41 +Strain SS/94)

To study the protective efficacy and the optimal immunization procedure of Newcastle disease, infectious bronchitis and avian influenza (H9 subtype) vaccine, inactivated Strain La Sota +Strain M41 +Strain SS/94 in the broiler of yellow feather chickens, the triple vaccine immunized yellow feather chickens by different immunization procedure. Results showed that the vaccinated chickens have the similar antibody variation to ND, IB and H9. The antibody titers were low when the triple inactivated vaccine were injected only once at 10 day-old. After the secondary and third immunization at the 20 or 40 day-old, or 10-day-old first with Newcastle disease virus (La Sota strain) and avian influenza virus (H9 subtype, SS/94 strain) vaccine, inactivated for primary immunization and then strengthened the immune with the triple inactivated vaccine at the 20 or 40 day-old, the antibody titers quickly ascended and maintained a long time. According to the results, proposed that on the basis of the normal immunization procedure, it may inject the triple inactivated vaccine at 20 day-old with 0.3 mL per chicken for short feeding period chickens and at 40 day-old with 0.5 mL per chicken for long feeding period chickens.