| 堆型艾美耳球虫子孢子纯化 |
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| 引用本文： | 黄芳,韦铮,王黎霞,安健.堆型艾美耳球虫子孢子纯化[J].北京农学院学报,2009,24(2). |
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| 作者姓名： | 黄芳 韦铮 王黎霞 安健 |
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| 作者单位： | 北京农学院动物科学技术系,北京,102206;北京农业职业学院,北京,102442 |
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| 基金项目： | 北京市教委资助项目，北京市属高校人才强教计划项目 |
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| 摘 要： | 笔者通过比较不同成分脱囊液的子孢子脱囊效果和不同蔗糖密度梯度分离纯化堆型艾美耳球虫子孢子的纯化效果来探索较佳的分离纯化堆型艾美耳球虫子孢子的条件。通过试验证明10%鸡胆汁+0.75%(W/V)胰酶脱囊效果最好。75%(W/V)和20%(W/V)蔗糖密度梯度离心得到的子孢子回收率最高,且纯度最好,染色证明纯化后的堆型艾美耳球虫子孢子仍具有较高的活性。
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| 关 键 词： | 堆型艾美耳球虫 子孢子纯化 梯度离心 |
The Purity of Eimeria acervulina Sporozoites |
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| HUANG Fang,WEI Zheng,WANG Li-xia,AN Jian.The Purity of Eimeria acervulina Sporozoites[J].Journal of Beijing Agricultural College,2009,24(2). |
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| Authors: | HUANG Fang WEI Zheng WANG Li-xia AN Jian |
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| Institution: | 1.Department of Animal Science and Veterinary Medicine;Beijing University of Agriculture;Beijing 102206;China;2.Beijing Vocational College of Agriculture Branch School in QingHe;Beijing 102208;China |
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| Abstract: | Through the intercomparison of excystation fluid with different components and different sucrose density gradients separation,optimized condition of Eimeria acervulina sporozoites purification was investigated.The study showed that 10% chicken bile and 0.75%(w/v)solution of trypsin had the best result;75% and 20% sucrose density gradients centrifugation obtained the best recovery rates and the purity of sporozoites,and the result of dying showed the activity of the sporozoties is very high. |
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| Keywords: | Eimeria acervulina sporozoites purification sucrose density gradients centrifugation |
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