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| 犬细小病毒VP_2基因原核表达载体的构建与分析 | |
| 引用本文： | 颜文卿,吴德峰,黄印尧,林永利,戴亚东,颜江华. 犬细小病毒VP_2基因原核表达载体的构建与分析[J]. 福建农林大学学报(自然科学版), 2006, 35(1): 60-62 |
| 作者姓名： | 颜文卿 吴德峰 黄印尧 林永利 戴亚东 颜江华 |
| 作者单位： | 1. 福建农林大学动物科学学院,福建,福州,350002;厦门大学抗癌研究中心,福建,厦门,361005 2. 福建农林大学动物科学学院,福建,福州,350002 3. 厦门出入境检验检疫局,福建,厦门,361012 4. 厦门大学抗癌研究中心,福建,厦门,361005 |
| 摘 要： | 从犬细小病毒/犬瘟热进口二联苗中提取犬细小病毒基因组DNA,以此为模板进行PCR扩增,PCR产物经BamH I和XhoI双酶切后,克隆至pET22b(+)质粒的相应位点上,构建了原核表达载体pET22b/VP2.重组质粒经限制性内切酶酶切和核苷酸序列分析表明,VP2基因已正确克隆到pET22b(+)载体上,从而成功地构建了pET22b/VP2表达载体. |
| 关 键 词： | 犬细小病毒 VP2基因 原核表达 载体构建 |
| 文章编号： | 1671-5470(2006)01-0060-03 |
| 收稿时间： | 2005-09-06 |
| 修稿时间： | 2005-12-05 |
Construction and analysis on prokaryotic expression vector of canine parvovirus VP2 gene |
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| YAN Wen-qing,WU De-feng,HUANG Yin-yao,LIN Yong-li,DAI Ya-dong,YAN Jiang-hua. Construction and analysis on prokaryotic expression vector of canine parvovirus VP2 gene[J]. Journal of Fujian Agricultural and Forestry University, 2006, 35(1): 60-62 | |
| Authors: | YAN Wen-qing WU De-feng HUANG Yin-yao LIN Yong-li DAI Ya-dong YAN Jiang-hua |
| Affiliation: | 1. College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2. Cancer Research Center, Xiamen University, Xiamen, Fujian 361005, China; 3. Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen, Fujian 361012, China |
| Abstract: | The genome DNA of canine parvovirus was extracted from CPV/CDV vaccine,which was used as templates for polymerase chain reaction(PCR) to amplify the VP_2 gene.Prokaryotic expression vector of pET22b/VP_2 was formed after PCR product was cloned into the restriction of plasmid pET22b( ) by endonuclease BamHI and XhoI process.In this experiment,recombinant plasmid pET22b/VP_2 was also identified by restriction enzymes analysis and nucleotide sequence analysis.The results showed that the VP_2 gene had been correctly cloned into plasmid pET22b( ). |
| Keywords: | canine parvovirus VP_2 gene prokaryotic expression vector construction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |