Molecular diagnosis of Marteilia sydneyi (Paramyxea) in Sydney rock oysters, Saccostrea commercialis (Angas) |
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| Authors: | T J ANDERSON R D ADLARD R J G LESTER |
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| Institution: | Department of Parasitology, The University of Queensland Brisbane, Queensland Australia |
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| Abstract: | Abstract. To prepare a diagnostic test, the polymerase chain reaction (PCR) with universal primers was used to amplify the internal transcribed spacer 1 (ITS1) of Marteilia sydneyi rDNA. A radiolabelled probe for M. sydneyi comprising putative ITS1 rDNA gave a strong signal for genomic DNA from M. sydneyi and no signal for host genomic DNA in Southern blots. A PCR to detect M. sydneyi used a forward primer designed from an internal site within the sequenced region and a reverse universal primer. It amplified a 650-bp fragment of M. sydneyi DNA when a combination of host and parasite DNA, extracted from an infected oyster, was used as the template. |
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